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Expression data of Oral squamous cell carcinoma (OSCC) cell lines CAL27, SCC4 and SCC25 treated with oral commensals

ABSTRACT: Oral bacterial species Streptococcus mitis, Neisseria flavescens, and Haemophilus parainfluenzae, and Porphyromonas gingivalis were each co-cultured with the OSCC cell lines CAL27, SCC4 and SCC25 individually. Total RNA was extracted from the cell lines, followed by gene expression profiling with microarray analysis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE183911 | GEO | 2021/09/11

REPOSITORIES: GEO

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Expression data of Oral squamous cell carcinoma (OSCC) cell lines CAL27, SCC4 and SCC25 treated with oral commensals

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Expression data from oral squamous cell carcinoma cell line Cal27 after lentivirus shIL-1beta transfection

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Project description:To study if non-invasive oral squamous cell carcinoma (OSCC) cells can acquire mechanical memory from stiff matrix and investigate the role of cell contractility in that process, Cal27 were conditioned (for 5 days) either by matrix stiffness or by direct modulation of cell contractility and then replated to a new niche lack of sources for the cells to learn. Cal27 with different types of conditioning were categorized either "Learn" or "Don't Lean/Forget" based on the existence of mechanical memory. Comparative gene expression profiling was done between "Learn" vs. "Don't Learn/Forget" Cal27 groups by RNA sequencing

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