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Induced intra- and intermolecular template switching as a therapeutic mechanism against RNA viruses

ABSTRACT: RNAseq experiments of Enterovirus A71 wild type demonstrate that the pyrazine-carboxamide ribonucleotide stimulates catalyzed intra- and intermolecular template switching. These results suggest that pyrazine-2 carboxamide ribonucleotides do not induce lethal mutagenesis or chain termination, but function by promoting template switching and formation of defective viral genomes. We conclude that RdRp-catalyzed intra- and intermolecular template switching can be induced by pyrazine-carboxamide ribonucleotides, defining an additional mechanistic class of antiviral ribonucleotides with potential for broad-spectrum activity.

ORGANISM(S): Homo sapiens

PROVIDER: GSE183959 | GEO | 2021/09/14

REPOSITORIES: GEO

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