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Single cell RNA sequencing to identify the PDGFR-β expressing lung cells

ABSTRACT: Purpose:Single cell RNA sequencing to identify PDGFR-beta expressing cells in the normal adult lung Methods: Adult Pdgfrb-CreERT2, ROSA26R(Zs/+) mice were induced with tamoxifen (1mg/day for 5 days) and rested for 5 days. Lungs were then harvested and prepared to obtain single cell suspension. Live Zs+ and Zs- cells were isolated by FACS and hash-tagged. Cells were then pooled together in PBS with 0.04% bovine serum albumin and subjected to library preparation and DropSeq. scRNA-seq data was processed with Cell Ranger v 3.1.0. Reads were aligned to a modified version of the mouse transcriptome mm10, including the sequence for the gene ZsGreen1. The top cell barcodes selected by Cell Ranger were utilized for downstream analysis. Results: In lung mesenchymal cells, populations of fibroblasts and pericytes express Pdgfr-beta.

ORGANISM(S): Mus musculus

PROVIDER: GSE184671 | GEO | 2021/09/23

REPOSITORIES: GEO

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