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RNAseq data for EndoC-βH1 treated with CM from omental stromal cells cultured either in the absence or presence of TNF-α or differentiated into adipocytes

ABSTRACT: We used an in vitro model to study the paracrine effect of stromal cells isolated from omental adipose tissue at different stages of differentiation and inflammation on the beta cell lines EndoC-βH1 through the use of conditioned media. We show that the expression of beta cells markers decreased and dedifferentiation markers increased when beta cells were cultured in conditioned medium derived from omental stromal cells. We report that stromal cells with a high pro-inflammatory profile had the most severe impact. By using RNAseq, we showed the stimulation of several signaling pathways such as STAT3, SMAD2 and RELA, as well as the downregulation of genes involved in lipogenesis and cholesterol synthesis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE184795 | GEO | 2021/09/28

REPOSITORIES: GEO

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