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Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids

ABSTRACT: Improved specification of metanephric nephron progenitors in conditions that prolong differentiation while simultaneously preventing spontaneous nephron induction resulted in proximal tubule (PT)-enhanced kidney organoids. PT-enhanced organoids exhibited improved PT maturation, with elongated and aligned nephron segments as well as distinct loops of Henle. The striking proximo-distal orientation of nephrons was shown to result from localised WNT antagonism originating from the centre of the organoid.

ORGANISM(S): Homo sapiens

PROVIDER: GSE184928 | GEO | 2022/08/25

REPOSITORIES: GEO

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Gene expression profiles in developing nephrons using Lim1 metanephric mesenchyme-specific conditional mutant mice

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