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Single cell RNA-seq analysis of mouse miR-205 inducible adult back skin

ABSTRACT: Through phenotypical study, we found that miR-205 can promote hair growth. The goal of this study is to determine how miR-205 effects epithelial cellular states and how miR-205 promotes hair growth at single cell level.

ORGANISM(S): Mus musculus

PROVIDER: GSE185115 | GEO | 2023/06/07

REPOSITORIES: GEO

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Transcriptome analysis in hair follicle stem cells after miR-205 induction in mouse

Project description:Through phenotypical study, we found that miR-205 can promote hair growth. The goals of this study is to identify direct targets of miR-205 in hair follicle stem cells and determine whether and how miR-205 promotes hair growth by activating hair follicle stem cell.

2023-06-07 | GSE185116 | GEO

RNA-seq for miR-205 inducible hair follicle stem cells and ScRNAseq for miR-205 inducible back skin samples

Project description:RNA-seq for miR-205 inducible hair follicle stem cells and ScRNAseq for miR-205 inducible back skin samples

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Expression data from miR-205 knockout in Hair Follicle Stem Cells (HFSCs) of mouse skin

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Expression data from miR-205 knockout in Hair Follicle Stem Cells (HFSCs) of mouse skin

2013-08-20 | E-GEOD-46447 | biostudies-arrayexpress

Single cell RNA-seq analysis of mouse miR-205 inducible adult back skin

Project description:Single cell RNA-seq analysis of mouse miR-205 inducible adult back skin

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Genes modulated by miR-205 in DU145 prostate cancer cells

Project description:The study was aimed at identifying genes directly or indirectly regulated by miR-205 in the prostate. To this purpose, DU145 prostate cancer cells, which express miR-205 at very low levels, were transfected with miR-205 synthetic precursor and consequent alterations of gene expression analyzed using a microarray approach. Keywords: comparison betweed cells exposed to different miRNA precursors

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RNA-seq for miR-205 inducible hair follicle stem cells and ScRNAseq for miR-205 inducible back skin samples

Project description:This SuperSeries is composed of the SubSeries listed below.

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Profiling of direct mRNA targets of miR-130a, miR-203 and miR-205 in prostate cancer cell line LNCaP

Project description:Micro RNAs (miRNAs) miR-130a, miR-203 and miR-205 are jointly downregulated in prostate cancer and act as repressors of AR-signaling. MiRNAs are small non-coding RNAs that regulate the expression of specific mRNA targets mainly by translational repression, mRNA deadenylation or cleavage. Reconstitution of these lost miRNAs in the LNCaP PCa cell line cause morphology changes, growth arrest, and apoptosis, increasing when the miRNAs were co-expressed. This series identifies direct targets of miR-130a, miR-203, and miR-205 by AGO2-RNA co-immunoprecipitation as described by (Beitzinger et al. 2007) upon miRNA reconstitution in LNCaP cells and analyzing AGO2-bound mRNAs using Affymetrix Genechips. Relative levels of AGO2 bound versus total RNA expression were compared between miRNA reconstituted and miR-scr transfected samples. Three arrays each for AGO2-bound RNA upon reconstitution of miR-130a, miR-203, miR-205, a scramble miRNA, and three arrays each for total RNA upon reconstitution of miR-130a, miR-203, miR-205, a scramble miRNA.

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Identification of miR-205 targets using an RIP-Chip assay with AGO2 antibody

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miR-205 is a critical regulator of lacrimal gland development

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