Transcriptomics

Dataset Information

0

Transcriptome sequencing Analysis of hLFSCs with and without mechanical stress


ABSTRACT: Purpose:The goals of this study are to MS (mechanical stress) how to change the cellular pathway of hLFSCs (human ligament flavum stem cells). Methods: Extract hLFSCs from the ligament flavum of patients.Treat the cells with and without (in control)MS.Total RNA was extracted from cells with Tripure Isolation Reagent.Subsequently, RNA was purified through rRNA depletion and then subjected to cDNA synthesis and RNA amplification. Next, random hexamer primer cDNA libraries were evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) and sequenced on Illumina Hiseq 4000 sequencing platform (Illumina, San Diego, California, USA) following the manufacturer’s instructions for paired-end 150 bp reads (Lifegenes, Shanghai, China).

ORGANISM(S): Homo sapiens

PROVIDER: GSE185614 | GEO | 2021/10/13

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2017-07-12 | GSE98034 | GEO
2017-12-01 | GSE101928 | GEO
2017-03-22 | GSE79484 | GEO
2018-10-29 | GSE111881 | GEO
2019-02-26 | GSE111127 | GEO
2021-03-04 | GSE164457 | GEO
2021-04-24 | GSE173202 | GEO
2022-01-31 | GSE194306 | GEO
2020-03-09 | GSE134322 | GEO
2022-05-13 | GSE196297 | GEO