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Murine C2C12 cells: GTP-stimulated cells vs. Control in DM

ABSTRACT: To define the signalling of extracellular GTP as enhancer of myogenesis, we investigated if the gene expression profile of differentiated C2C12 cells (4 hours in DM) was affected by extracellular GTP.

ORGANISM(S): Mus musculus

PROVIDER: GSE18584 | GEO | 2009/10/29

SECONDARY ACCESSION(S): PRJNA120295

REPOSITORIES: GEO

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Murine C2C12 cells: GTP-stimulated cells vs. Control in DM

Project description:To define the signalling of extracellular GTP as enhancer of myogenesis, we investigated if the gene expression profile of differentiated C2C12 cells (4 hours in DM) was affected by extracellular GTP. Two-condition experiment, GTP-treated C2C12 cells vs Control C2C12 cells at 4 h of differentiation. Biological replicates: 1 control, 1 GTP-treated cells, independently grown and harvested. One replicate per array.

2010-06-20 | E-GEOD-18584 | biostudies-arrayexpress

Murine C2C12 cells: GTP-stimulated cells vs. Control in SM

2009-10-29 | GSE18531 | GEO

Murine C2C12 cells: GTP-stimulated cells vs. Control in SM

Project description:To define the signalling of extracellular GTP as enhancer of myogenesis, we investigated if the gene expression profile of differentiated C2C12 cells (4 and 24 hours in SM) was affected by extracellular GTP. Two-condition experiment, GTP-treated C2C12 cells vs Control C2C12 cells at 4 h and 24 h of differentiation. Biological replicates: 1 control, 1 GTP-treated cells, independently grown and harvested. One replicate per array.

2010-06-20 | E-GEOD-18531 | biostudies-arrayexpress

Proteomic and phosphoproteomic of C2C12 myogenesis and MAPK inhibition

Project description:Myogenesis is governed by signalling networks whose regulations are tightly controlled in a time-dependent manner. While different protein kinases have been identified to regulate various aspects of myogenesis, knowledge on the global signalling networks and their downstream substrates during myogenesis remains incomplete. Here, we map the myogenic differentiation of C2C12 cells using mass spectrometry (MS)-based phosphoproteomics and proteomics. From these data, we infer global kinase activity and predict substrates of key kinases that are involved in myogenesis. We found that multiple mitogen-activated protein kinases (MAPKs) mark the initial wave of signalling cascades. Further phosphoproteomic and proteomic profiling with MAPK1/3 and MAPK8/9 specific inhibitions unveil their shared and distinctive roles on myogenesis.

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shRNA of mouse C2C12 culture: myogenesis timecourse and shRp58 treatment

Project description:To predict Rp58-regulated gene involved in myogenesis, RNA profiling experiments were performed, comparing RNA derived from C2C12 with or without expressing shRNA for Rp58. As a result, 271 genes were upregulated in C2C12 stably expressing shRNA-Rp58 cells compared with control C2C12 cells. As Rp58 is repressor in C2C12, we hypothesized that Rp58 regulates gene cluster which expression is downregulated in accordance with Rp58 expression and myogenesis progression. In this regard, we also characterized dynamic gene expression patterns during myogenesis by microarray at 4 different stage (GM, day 0, 2, 4) of C2C12 myogenesis assays and found that 399 genes expression is characterized as downregulation pattern during myogenesis. Importantly, this down regulation gene set and upregulated genes by shRNA for Rp58 were highly overlapped. Experiment Overall Design: C2C12 murine skeletal muscle cells were purchased from American Type Experiment Overall Design: culture Collection (ATCC). These cells were mainteined in GM (DMEM Experiment Overall Design: supplemented with 10% FBS). Cells were grown in GM and after reaching Experiment Overall Design: counfluence, the medium was switched to DM (DMEM supplemented with 2% hourse serum) and further incubated. The medium was changed every 2 days. Culture was performed by using within five passages cells. For the experiment of shRNA for Rp58, transfection was performed by using Lipofectamin 2000 (Invitrogen). Stable transfectants were obtained by selection of the transfected C2C12 cells for two weeks. Experiment Overall Design: Microarray analysis - RNA was isolated as described from C2C12, and cRNA was synthesized. 10 ug of cRNA were hybridized to Affymetrix mouse 430 2.0 arrays. Intensity values were quantified using RMA algorithm. Experiment Overall Design: MAPPFinder (www.genmapp.org) was used to integrate expression data with known pathways.

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C2C12 culture: myogenesis timecourse and shRp58 treatment

2009-07-31 | GSE12993 | GEO

Phosphoproteomics of C2C12 differentiation

Project description:Phosphoproteomic analysis of myogenesis in C2C12 myoblasts differentiating into myotubes. Four time points were analysed (0min, 30min, 24h and 5d) with four biological replicates.

2024-01-26 | PXD023413 | Pride

Genome-wide occupancy of LSD1 in differentiating C2C12 myoblasts.

Project description:ChIP-seq analysis of LSD1 in C2C12 cells. We found that LSD1 directly regulates the expression of fiber and metabolism genes during myogenesis. Results provide insight into the molecular mechanisms of myogenesis.

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Transcriptomic profiling of wild-type C2C12 cells and C2C12 cells with Myod1 gene knockout by RNA-sequencing

Project description:C2C12 is a myoblast cell line usually used for study of muscle differentiation. Myod1 is a pro-differentiation factor for myogenesis. We found that CRISPR/Cas9-mediated Myod1 gene knockout in C2C12 cells led to the loss of myoblast identity and gain of neural properties.

2021-01-27 | GSE137507 | GEO

miRNA expression profiling during C2C12 myogenesis

Project description:This study profiled the expression of miRNA during myogenesis of the C2C12 mouse myoblast cell line. Cells were harvest at different stages during myogenesis including proliferating, confluence, 1 day post-differentiation induction, 2 day post-differentiation induction and 4 days post-differentiation induction. Keywords: time course

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