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Epigenetic signature induced by prolonged culture may impact therapeutic function of Tregs used in adoptive cell therapy

ABSTRACT: Adoptive transfer of regulatory T cells (Treg) is a promising new therapeutic option to treat detrimental inflammatory conditions. To reach sufficient cell yield for treatment, ex vivo isolated autologous or allogenic Tregs need to be expanded extensively in vitro during manufacturing of the Treg product. However, repetitive cycles of restimulation and prolonged culture have been shown to impact T cell phenotypes, functionality and fitness. We performed genome-wide DNA methylation profiling of cells throughout the manufacturing process of a polyclonal Treg product, which may be informative for predicting the cellular state and function of the final Treg product.

ORGANISM(S): Homo sapiens

PROVIDER: GSE185854 | GEO | 2021/10/15

REPOSITORIES: GEO

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