Stimulations of the growth rate in elevated CO2 condition were observed in over-expression or RNA interference lines of genes identified by intraspecific variation analyses.

ABSTRACT: In the present study, we sought genes that are involved in the variation of the enhancement of plant growth rate among the Arabidopsis thaliana ecotypes in elevated CO2 condition. We performed a combination analysis of a genome wide association study (GWAS), and a transcriptome study to seek the candidate genes. GWAS was performed using the growth analysis data obtained at Oguchi et al. and the whole genome data from 31 ecotypes. The transcriptome analysis was performed for 6 ecotypes that had contrasting CO2 responses in the growth rate. We analyzed not only annotated coding genes but also small coding genes (30–100 amino acids) identified by Hanada et al., most of which were shown to play roles in cell-to-cell signaling, because it has been shown that a number of small coding genes play significant roles in environmental responses. We also studied the effect of the altered expression of the candidate genes on the plant growth rate in elevated CO2 condition using over-expression (OX) transgenics and RNA interference (RNAi) transgenics. We show that transgenic plants of three genes had significantly higher growth rate under the elevated CO2 condition.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE185907 | GEO | 2021/10/16

REPOSITORIES: GEO