Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profiling in PA1 ovarian cancer cells under anchorage independence either in the presence of vehicle or BMP9 treatment for 24 hours

ABSTRACT: BMP9 promotes anoikis sensitivity in cancer cell lines. Here we examine the changes in gene expression in response to BMP9 in cells under anoikis to identify potential pathways associated with anoikis sensitivity.

ORGANISM(S): Homo sapiens

PROVIDER: GSE185924 | GEO | 2022/07/26

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression profiling in PA1 ovarian cancer cells under anchorage independence either in the presence of vehicle or BMP9 treatment for 24 hours

Project description:Gene expression profiling in PA1 ovarian cancer cells under anchorage independence either in the presence of vehicle or BMP9 treatment for 24 hours

| PRJNA771377 | ENA

Pro-BMP9 and sENG monomer:BMP9 complex signalling in human pulmonary arterial endothelial cells after 1.5 hours treatment

Project description:This experiment was desinged to investigate the difference in global gene expression induced by the circulating form of BMP9, pro-BMP9, and BMP9 in complex with soluble endoglin (sENG:BMP9).

2019-09-23 | GSE119206 | GEO

Energy Metabolism during Anchorage-Independence

Project description:The detachment of epithelial cells, but not cancer cells, causes anoikis due to reduced energy production. Invasive tumor cells generate three splice variants of the metastasis gene osteopontin. The cancer-specific form osteopontin-c supports anchorage-independence through inducing oxidoreductases and upregulating intermediates/enzymes in the hexose monophosphate shunt, glutathione cycle, glycolysis, glycerol phosphate shuttle, and mitochondrial respiratory chain. Osteopontin-c signaling upregulates glutathione (consistent with the induction of the enzyme GPX-4), glutamine and glutamate (which can feed into the tricarboxylic acid cycle). Consecutively, the cellular ATP levels are elevated. The elevated creatine may be synthesized from serine via glycine and also supports the energy metabolism by increasing the formation of ATP. Metabolic probing with N-acetyl-L-cysteine, L-glutamate, or glycerol identified differentially regulated pathway components, with mitochondrial activity being redox dependent and the creatine pathway depending on glutamine. The effects are consistent with a stimulation of the energy metabolism that supports anti-anoikis. Our findings imply a synergism in cancer cells between osteopontin-a, which increases the cellular glucose levels, and osteopontin-c, which utilizes this glucose to generate energy. mRNA profiles of MCF-7 cells transfected with osteopontin-a, osteopontin-c and vector control were generated by RNA-Seq, in triplicate, by Illumina HiSeq.

2014-02-21 | E-GEOD-55193 | biostudies-arrayexpress

Energy Metabolism during Anchorage-Independence

2014-02-21 | GSE55193 | GEO

Pro-BMP9 signalling in human pulmonary arterial endothelial cells after 5 hours treatment

Project description:This study was designed to investigate the global gene expression of circulating, prodomain bound form of BMP9 (pro-BMP9) in human pulmonary arterial endothelial cells (PAECs) because previous microarray experients have used the growth factor domain alone. We are interested in its signalling capacity in pulmonary vascular endothelial cells using the physiological circulating form and at physiologically circulating concentrations. Also, this study reports data 5 hours after the treatment to look at the secondary genes regulated by BMP9 signalling.

2021-08-08 | GSE118353 | GEO

Expression profiling of BMP9-stimulated mesenchymal stem cells

Project description:To identify potential target genes regulated by BMP9 in MSCs, we used microarray to profile expression patterns of BMP9- vs. GFP-stimulated MSCs.

2013-07-16 | GSE48882 | GEO

Expression profiling of BMP9-stimulated mesenchymal stem cells

Project description:To identify potential target genes regulated by BMP9 in MSCs, we used microarray to profile expression patterns of BMP9- vs. GFP-stimulated MSCs. Subconfluent mouse C3H10T1/2 cells were transduced with adenoviral vector expressing BMP9 or GFP for 30h. Total RNA was isolated for microarray analysis using Affymetrix mouse MOE430A GeneChips. Experiments were done in triplicate.

2013-07-16 | E-GEOD-48882 | biostudies-arrayexpress

BMP9 signaling promotes the normalization of tumor blood vessels

Project description:We sought to evaluate the effects of BMP9 on tumor vascular formation. We observed that BMP9 promotes vascular normalization with decreased permeability and increased perfusion and perivascular cell coverage. Gene expression analysis of CD31-positive cells isolated from control and BMP9-overexpressing tumors revealed enrichment of pathways involved in Notch signaling and vascular quiescence.

2020-01-26 | GSE144256 | GEO

Transcriptional profiling of mouse aorta after deletion of BMP9 and BMP10

Project description:Aorta was isolated from ANF-Cre positive/BMP10 loxP/loxP/BMP9-/- (BMP9/10 dKO) mice. ANF-Cre negative BMP10 loxP/loxP mice were used as controls. Transcritional profiling was performed to understand the impact of BMP9/10 expression on aorta function.

2021-02-05 | GSE160026 | GEO

Transcriptional profiling of mouse aorta after deletion of BMP9 and BMP10.

Project description:Aorta was isolated from ANF-Cre positive / BMP10 loxP/loxP / BMP9-/- (BMP9/10 dKO) mice. ANF-Cre negative BMP10 loxP/loxP mice were used as controls. Transcriptional profiling was performed to understand the importance of BMP9/10 expression on aorta function.

2020-10-30 | E-MTAB-8914 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data