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RNA-seq in HCT116 cell lines

ABSTRACT: Purpose: The goals of this study are to invsetigate the primary biological process of hnRNPK. Methods: Briefly, samples (HCT116 cells transfected with control or hnRNPK shRNA) were used to extract total RNAs for quality control and RNA-seq loading samples. RNA-seq was performed as DGE on an Illumia HiSeq platform and 50 bp paired-end reads were generated (BGI Co. Ltd.). Results: Regulation of transcription by RNA polymerase II was identified as the primary biological process according to the gene ontology (GO) analysis. Regulation of transcription by RNA polymerase II and translation by ribosome were identified as the primary biological processes according to the gene set enrichment analysis (GSEA). Conclusions: RNA-seq in HCT116 cell lines revealed that hnRNPK mainly regulated cellular transcription and translation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE186371 | GEO | 2021/10/24

REPOSITORIES: GEO

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