Transcriptomics

Dataset Information

0

Single cell multiomic readouts reveal that cellular heterogeneity impacts lipid nanoparticle-mediated mRNA delivery


ABSTRACT: We report the development of a new multiomic nanoparticle delivery system called Single cEll Nanoparticle Transcriptome-sequencing (SENT-seq), which quantifies how dozens of lipid nanoparticles (LNPs) deliver DNA barcodes and mRNA into cells, subsequent protein production, and the transcriptome, with single cell resolution. We show from the sequencing data that cell heterogeneity influences the efficiency with which LNPs deliver mRNA therapies, and identify cell subtypes that exhibit particularly high or low LNP uptake as well as genes associated with those subtypes. These data suggest that cell subsets have distinct responses to LNPs, and that these differential interactions can affect mRNA therapies.

ORGANISM(S): Mus musculus

PROVIDER: GSE186395 | GEO | 2023/01/23

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2023-05-15 | GSE220777 | GEO
2024-11-12 | GSE249674 | GEO
2024-10-14 | GSE279372 | GEO
2021-08-04 | GSE181333 | GEO
2024-12-06 | GSE280933 | GEO
2023-09-05 | PXD041648 | Pride
2023-07-20 | PXD043932 |
2017-04-26 | GSE98211 | GEO
2021-03-16 | GSE167521 | GEO
2024-08-16 | GSE243992 | GEO