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Transcriptomic analysis of MAPK KOs mutants under normal growing conditions (bb)

ABSTRACT: au07-10_mpk - bb - Gain insight into the function of mpk genes by characterizing the transcriptional profile of the mpk5, mpk10 and mpk11 KO mutants in comparison with that of the Arabidopsis wt plants. - Transcriptomic analysis of the double mutant bak1-4 bkk1-1 to compare its gene expression profile with that of the single mutants atmekk1 and atmpk4 and elucidate their involvement in the brassinolide signalling. Keywords: gene knock out

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE18748 | GEO | 2009/10/30

SECONDARY ACCESSION(S): PRJNA121663

REPOSITORIES: GEO

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Transcriptomic analysis of MAPK KOs mutants under normal growing conditions (bb)

Project description:au07-10_mpk - bb - Gain insight into the function of mpk genes by characterizing the transcriptional profile of the mpk5, mpk10 and mpk11 KO mutants in comparison with that of the Arabidopsis wt plants. - Transcriptomic analysis of the double mutant bak1-4 bkk1-1 to compare its gene expression profile with that of the single mutants atmekk1 and atmpk4 and elucidate their involvement in the brassinolide signalling. Keywords: gene knock out 1 dye-swap - CATMA arrays

2009-10-30 | E-GEOD-18748 | biostudies-arrayexpress

Transcriptomic analysis of MAPK KOs mutants under normal growing conditions (mpk)

Project description:au07-10_mpk - mpk - Gain insight into the function of mpk genes by characterizing the transcriptional profile of the mpk5, mpk10 and mpk11 KO mutants in comparison with that of the Arabidopsis wt plants. - 1) mpk5 (WS genetic background) versus wt-WS 2) mpk11 (Col0 genetic background) versus wt-Col0 3) mpk10 (Col0 genetic background) versus wt-Col0 Seeds were surface sterilized and grown in MS agar medium (3% sucrose) for 11 days in long day conditions. Entire seedling were harvested and deep frozen in liquid nitrogen. Keywords: gene knock out

2009-10-30 | GSE18749 | GEO

Transcriptomic analysis of MAPK KOs mutants under normal growing conditions (mpk)

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Human fibroblast stimulation with PDGF-BB or b-FGF

Project description:We analyzed gene expression in human fibroblasts stimulated by platelet-derived growth factor-BB (PDGF-BB) or basic fibroblast growth factor (bFGF) for 1h and 24h. The results of two independent experiments were merged. SAM analysis identified 116 relevant probe sets. Hierarchical clustering of these probe sets showed divergent early gene regulation by PDGF and FGF but overlapping late response. We first analyzed genes commonly regulated by PDGF-BB and b-FGF more than 2 fold after 24h of stimulation and we found that these two growth factors repressed FOXO. We then focused on the early gene expression response induced by both growth factors. We performed a fold change analysis and found 114 probe sets regulated by PDGF-BB and 42 probe sets regulated by b-FGF, 37 of which were shared between the two gene lists . Keywords: Time course, cell Treatment comparison

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