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MicroRNA expression profiling in adult mouse liver following benzo(a)pyrene (BaP) treatment [Agilent miRNA array]

ABSTRACT: Lack of change in microRNA expression in adult mouse liver following treatment with benzo(a)pyrene (BaP), as detected using Agilent miRNA arrays. We have investigated the effect of exposure to 150 mg/kg benzo(a)pyrene (BaP) for 3 days on mRNA and miRNA expression levels in adult mouse liver. We used Agilent miRNA array platforms to assess effects of BaP exposure on miRNA expression levels. Our results indicate a distinct lack of effect of BaP of miRNA expression, despite widespread changes in mRNA levels. Keywords: Toxicology, miRNA

ORGANISM(S): Mus musculus

PROVIDER: GSE18786 | GEO | 2010/10/18

SECONDARY ACCESSION(S): PRJNA123861

REPOSITORIES: GEO

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MicroRNA expression profiling in adult mouse liver following benzo(a)pyrene (BaP) treatment [Exiqon miRNA array]

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MicroRNA expression profiling in adult mouse liver following benzo(a)pyrene (BaP) treatment [Exiqon miRNA array]

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Reference: MicroRNA expression profiling in adult mouse liver following BaP treatment [Agilent miRNA array]

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Toxicogenomics and phenotypic characterization of adult male zebrafish liver treated with benzo-[A]-pyrene (BAP)

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mRNA expression profiling in adult mouse liver following benzo(a)pyrene (BaP) treatment [Agilent gene expression array]

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2010-10-18 | E-GEOD-18789 | biostudies-arrayexpress

Toxicogenomics and phenotypic characterization of adult male zebrafish liver treated with benzo-[A]-pyrene (BAP)

2012-01-01 | E-GEOD-30035 | biostudies-arrayexpress

Reference: MicroRNA expression profiling in adult mouse liver following BaP treatment [Agilent miRNA array]

Project description:We have investigated the effect of exposure to 150 mg/kg benzo(a)pyrene (BaP) for 3 days on mRNA and miRNA expression levels in adult mouse liver. We used Agilent miRNA array platforms to assess effects of BaP exposure on miRNA expression levels. Our results indicate a distinct lack of effect of BaP of miRNA expression, despite widespread changes in mRNA levels. The data in the attached array files were used a positive control for the Agilent platform, to indicate that the platform was able to detect significant differences in abundance of miRNA between two samples with great differences in miRNA content. Keywords: Toxicology, miRNA Two reference pools were created from commercially available reference RNA (FirstChoice® mouse Total RNA including the small fraction (Catalogue # AM7800-AM7828, Ambion, Streetsville, ON)). Reference #1 contained equal parts of mouse testicle, ovary and 10-12 day embryo. Reference #2 contained liver, heart and lung. These references were hybridized to a single mouse Agilent 8x15K miRNA array (4 replicates each), normalized by cyclic lowess and analysed with MAANOVA to find differences in the samples.One reference #1 sample was not included in the analysis because it did not pass Agilent quality control metrics.

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Lack of hepatic response of microRNA in mice following chronic benzo(a)pyrene exposure (miRNA)

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