Transcriptomics

Dataset Information

0

Characterisation of iPSC derived Sensory neurons with Single Cell RNA-seq


ABSTRACT: Despite the rapid development of protocols to differentiate human pluripotent stem cells (hPSCs) into neurons, those used to produce sensory neurons remain difficult to replicate and result in heterogenous populations. Moreover, there is a growing clinical burden of chronic pain conditions, highlighting a need for relevant human cellular models. This study presents a hybrid differentiation method to produce nociceptive sensory neurons from hPSCs. Cell lines harbouring an inducible NEUROG2 construct were patterned towards precursors with small molecules, followed by NEUROG2 overexpression. We profilled cells with single cell RNA sequencing, which revealed populations of nociceptors, demonstrated by the expression of key developmental and fucntional genes including volatge gated sodium and TRP channels. Overall, this study represents an optimisation of existing methods to efficiently produce nociceptive sensory neurons and provides a tool for chronic pain research and the development of treatments.

ORGANISM(S): Homo sapiens

PROVIDER: GSE188272 | GEO | 2022/11/17

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2017-05-25 | GSE99265 | GEO
2011-04-01 | E-GEOD-26867 | biostudies-arrayexpress
2024-06-20 | GSE261529 | GEO
2020-03-02 | GSE131619 | GEO
2020-03-02 | GSE131622 | GEO
2020-03-02 | GSE131621 | GEO
2022-06-24 | GSE192566 | GEO
2013-11-01 | GSE25611 | GEO
2022-11-17 | GSE187345 | GEO
2023-08-05 | GSE236865 | GEO