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Let-7c and miR-294 target identification in mouse ES cells

ABSTRACT: let-7c and miR-294 were transfected into Dgcr8 -/- miRNA deficient ES cells and RNA was harvested after 12 hours the goal of this study was to identify direct and indirect targets of the let-7c and miR-294 miRNAs, we chose to harvest RNA early at 12 hours to mimize secondary effects due to ES cell differentiation, prior to performing the array experiment we found that at this time point candidate miRNA targets were maximally downregulated by qPCR

ORGANISM(S): Mus musculus

PROVIDER: GSE18840 | GEO | 2010/01/01

SECONDARY ACCESSION(S): PRJNA121175

REPOSITORIES: GEO

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