Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Stella regulates the development of female germline stem cells by modulating chromatin structure and DNA methylation [Hi-C]

ABSTRACT: We use in situ Hi-C to report the most comprehensive survey to date of chromatin organization in female germline stem cells

ORGANISM(S): Mus musculus

PROVIDER: GSE188661 | GEO | 2022/05/25

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Stella regulates the development of female germline stem cells by modulating chromatin structure and DNA methylation [Hi-C_2]

Project description:We use in situ Hi-C to report the most comprehensive survey to date of chromatin organization in female germline stem cells

2022-05-25 | GSE199075 | GEO

Stella regulates the development of female germline stem cells by modulating chromatin structure and DNA methylation [ChIP-Seq]

Project description:We use ChIP-seq to report the most comprehensive survey to date of chromatin organization in female germline stem cells

2022-05-25 | GSE188660 | GEO

3D Chromosome Structure of SSCs, iGSCs and FGSCs

Project description:We use in situ Hi-C to report the most comprehensive survey to date of chromatin organization in germline stem cells

2022-07-29 | GSE135104 | GEO

3D Chromosome Structure Can Reveal Stem Cell Signatures (Hi-C)

Project description:We use in situ Hi-C to report the most comprehensive survey to date of chromatin organization in stem cells

2022-01-20 | GSE126011 | GEO

Metformin promotes female germline stem cell proliferation by upregulating Gata-binding protein 2 with histone β-hydroxybutyrylation [ChIP-seq]

Project description:We use ChIP-seq to report the most comprehensive survey to date of chromatin organization in female germline stem cells

2023-04-26 | GSE217782 | GEO

<i>Stella</i> Regulates the Development of Female Germline Stem Cells by Modulating Chromatin Structure and DNA Methylation.

Project description:Female germline stem cells (FGSCs) have the ability to self-renew and differentiate into oocytes. Stella, encoded by a maternal effect gene, plays an important role in oogenesis and early embryonic development. However, its function in FGSCs remains unclear. In this study, we showed that CRISPR/Cas9-mediated knockout of Stella promoted FGSC proliferation and reduced the level of genome-wide DNA methylation of FGSCs. Conversely, Stella overexpression led to the opposite results, and enhanced FGSC differentiation. We also performed an integrative analysis of chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq), high-throughput genome-wide chromosome conformation capture (Hi-C), and use of our published epigenetic data. Results indicated that the binding sites of STELLA and active histones H3K4me3 and H3K27ac were enriched near the TAD boundaries. Hi-C analysis showed that Stella overexpression attenuated the interaction within TADs, and interestingly enhanced the TAD boundary strength in STELLA-associated regions. Taking these findings together, our study not only reveals the role of Stella in regulating DNA methylation and chromatin structure, but also provides a better understanding of FGSC development.

| S-EPMC9066111 | biostudies-literature

3D chromosome structure reveals unique signatures involved in mouse female germline stem cells and their development

Project description:We use in situ Hi-C to report the female germline cell development

2022-01-20 | GSE137771 | GEO

Stella regulates the development of female germline stem cells by modulating chromatin structure and DNA methylation

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-05-25 | GSE188961 | GEO

3D Chromosome Structure Can Reveal Stem Cell Signatures (RNA-Seq)

Project description:We use in situ Hi-C to report the most comprehensive survey to date of chromatin organization in stem cells

2022-01-20 | GSE126013 | GEO

3D Chromosome Structure Can Reveal Stem Cell Signatures (ChIP-Seq)

Project description:We use in situ Hi-C to report the most comprehensive survey to date of chromatin organization in stem cells