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Time-regulated transcripts with the potential to modulate human pluripotent stem cell-derived cardiomyocyte differentiation [miRNA]

ABSTRACT: Human pluripotent stem cell-derived cardiomyocytes (hPSC-CM) are a promising disease model, although hPSC-CMs are not fully mature cardiomyocytes. We examined exponential differentially expressed miRNA/gene to identify critical time-regulated transcripts associated with hPSC-CM differentiation. We uncovered 324 interactions among 29 differentially expressed genes and 51 miRNAs from 20.543 transcripts during the 120 days of hPSC-CM differentiation. We found 16 genes and 26 miRNAs with an inverse pattern of expression (Pearson R-values < -0.5) validated using several human and mouse databases. We further validated these findings using two hPSC-CM lines and seven sampling times over a 30-day protocol and observed 16 inverse interactions among eight genes and 12 miRNAs (Person R-values < -0.5) changing over time. Finally, we tested the top eight miRNAs by adding miRNA mimics to differentiating hPSC-CMs and found that they influenced proliferation and maturation phenotypes. These time-regulated transcripts appear to regulate single or multiple pathways affecting cardiac differentiation.

ORGANISM(S): synthetic construct Homo sapiens

PROVIDER: GSE188731 | GEO | 2022/09/15

REPOSITORIES: GEO

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Time-regulated transcripts with the potential to modulate human pluripotent stem cell-derived cardiomyocyte differentiation [gene]

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