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Single-cell analysis reveals an indispensable role of SRSF2 in controlling fate of Myf5-expressing cells during skeletal muscle development

ABSTRACT: Skeletal muscle is an important tissue of animal body, which plays an important role in the process of motion, support and energy metabolism. Skeletal muscles are composed of striated myofibers bundles which formed during embryonic myogenesis and may undergo remodeling in the process of muscle regeneration after birth. SRSF2 is an important member of the splicing factor SR protein family. It plays an active role in transcription elongation, coupling transcription and splicing. A large number of studies have shown that it plays a key role in maintaining the normal development of thymus, heart and bone marrow. Here, we have used single cell RNA-seq and lineage analysis and gene knockout animal model to explore the regulation of splicing factor SRSF2 on myoblast cell fate and differentiation, as well as the mechanism in mouse skeletal muscle development.

ORGANISM(S): Mus musculus

PROVIDER: GSE189088 | GEO | 2022/04/21

REPOSITORIES: GEO

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