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Circular RNA expression in the rat orbitofrontal cortex after self-administration of heroin or sucrose

ABSTRACT: In this study we identified circular RNAs that are regulated in the orbitofrontal cortex after chronic heroin self-administration of a dosage of 0.03mg/kg/infusion or sucrose self-administration.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE189192 | GEO | 2022/02/16

REPOSITORIES: GEO

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circular RNA expression in the rat orbitofrontal cortex after self-administration of heroin or sucrose

Project description:circular RNA expression in the rat orbitofrontal cortex after self-administration of heroin or sucrose

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Project description:Noncoding RNAs, especially microRNAs (miRNAs) have been implicated in the regulation of neuronal functions, such as learning, cognition and memory formation. However, the particular miRNAs involved in drug-induced behavioral plasticity are largely unknown. Here we report a novel regulator, miR-218, that inhibits heroin-induced behavioral plasticity. Network propagation-based method revealed several miRNAs that play key roles in drug-addiction, among which, miR-218 was decreased in nucleus accumbens (NAc) after chronic exposure to heroin. Lentiviral overexpression of miR-218 in NAc could inhibit heroin-induced reinforcement in both conditioning place preference (CPP) test and heroin self-administration (SA) experiment. Luciferase activity assay indicated miR-218 could regulate neuroplasticity related genes and directly target Mecp2 3’UTR. Consistently, Mecp2-/y mice exhibited reduced heroin seeking behavior in CPP test. These data reveal a functional role of miR-218 and its target, Mecp2, in the regulation of heroin-induced behavioral plasticity.

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Heroin Regulates Orbitofrontal Circular RNAs.

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