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ScRNA-Seq and snRNA-Seq in human adipose tissue

ABSTRACT: From subcutaneous adipsoe tissue from 2 particpants we performed scRNA-seq on the stromal vascular fraction, snRNA-seq on isolated adipocytes and snRNA-seq on frozen adipose tissue. We performed clustering analysis, differential gene expression analysis and over-representation analysis to decipher the different cell populations detectable with each technique. The SVF fraction was able to show more diverse immune cell populations. Whilst the snRNA-seq frozen adipose tissue showed diverse clusters of adipocytes that was not observable from a snRNA-Seq fraction

ORGANISM(S): Homo sapiens

PROVIDER: GSE189346 | GEO | 2022/08/01

REPOSITORIES: GEO

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