Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression analysis in lymphocyte subsets from mice injected with control- or leukemia-derived extracellular vesicles

ABSTRACT: mRNA profiles of Treg, CD4+, CD8+, CD19+ splenic cells from 8-week-old Foxp3 YFP/cre mice injected with control (WT) or leukemic (TCL1) extracellular vesicles (EV) were analyzed in triplicate.

ORGANISM(S): Mus musculus

PROVIDER: GSE189391 | GEO | 2022/08/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression analysis in murine lymphocyte subsets incubated with control or leukemia-derived extracellular vesicles

Project description:mRNA profiles of Treg, CD4+, CD8+, CD19+ splenic cells from 8-week-old Foxp3 YFP/cre mice incubated with control (WT) or leukemic (TCL1) extracellular vesicles (EV) were analyzed in triplicate.

2022-08-31 | GSE188898 | GEO

Expression data from CD19-positive splenic B cells isolated from 1-month old ID4+/-TCL1-tg and ID4+/+TCL1-tg mice

Project description:The function of ID4 in CLL development was studied in vivo using TCL1 transgenic mouse model that develop leukemia similar to human CLL. TCL1 mice with ID4 single knockout gene have accelerated CLL progression. Results from the animal study suggest ID4 as a tumor suppressor gene that might regulate cell proliferation and apoptosis in B lymphocytes. Gene expression in CD19-positive splenic B cells collected from 1-month old ID4+/-TCL1-tg and ID4+/+TCL1-tg mice was compared by microarray, the goal is to find ID4-regulated genes involved in CLL development.

2010-11-04 | E-GEOD-25100 | biostudies-arrayexpress

Gene expression in murine CD8+ T cells treated with leukemia extracellular vesicles

Project description:mRNA profiles of CD8+ T cells from 8-week-old wild-type (Foxp3 YFP/cre) mice treated with control or leukemic sEV were generated by 3'-sequencing, in triplicate.

2022-08-31 | GSE190789 | GEO

Integrin activation controls regulatory T cell-mediated peripheral tolerance

Project description:RNA sequencing analysis of Foxp3+YFP+ talin KO Treg cells compared to Foxp3+YFP+ wild-type Treg cells isolated from the spleen of 1-2 individual animals.

2018-09-13 | GSE111791 | GEO

Gene expression in murine T cells from Ebi3-WT or Ebi3-KO mice

Project description:mRNA profiles of T cells from 8-week-old wild-type (Foxp3 YFP/cre) or Ebi3-KO (Foxp3 YFP/cre Ebi3-/-) mice were generated by 3'-sequencing, in triplicate.

2023-05-31 | GSE216131 | GEO

Mus musculus

Project description:Gene expression profile of splenic CD19 cells from NTg, PTPROt Tg, TCL1 Tg and PTPROt/TCL1 double Tg mice

| PRJNA258585 | ENA

NF-κB-induced expression of T-bet suppresses the proliferation of malignant B cells and is associated with good prognosis in chronic lymphocytic leukemia

Project description:Tbx21-/- TCL1 cells were generated to explore the effect of T-bet expression in malignant B cells. Single-cell suspensions from spleens of Tbx21 knockout and control TCL1 mice were prepared and splenocytes were enriched in TCL1 cells, resulting in a purity above 97% of CD5+ CD19+ cells. RNA sequencing libraries were prepared using the Illumina TruSeq Stranded mRNA Kit and sequenced on the Illumina NovaSeq 6000.

2024-01-31 | E-MTAB-13030 | biostudies-arrayexpress

Regulatory T cells from colonic lamina propria [RNA-Seq]

Project description:The colonic lamina propria contains a distinct population of Foxp3+ T regulatory cells (Tregs) that modulate responses to commensal microbes. Analysis of gene expression revealed that the transcriptome of colonic Tregs is distinct from splenic and other tissue Tregs. Rorγ and Helios in colonic Tregs mark distinct populations: Rorγ+Helios- or Rorγ-Helios+ Tregs. We uncovered an unanticipated role for Rorγ, a transcription factor generally considered to be antagonistic to Foxp3. Rorγ in colonic Tregs accounts for a small but specific part of the colon-specific Treg signature. Nrp1- Tregs were sorted from Foxp3-cre.Rorcfl/fl mice, which have a Treg-selective deletion of Rorc, or paired WT littermates. For low-input RNAseq, 1,000 TCRb+CD4+YFP(Foxp3)+Nrp1- cells were double-sorted into Trizol, RNA extracted and reverse-transcribed using ArrayScript (Ambion). To reduce variability at least three replicates were generated.

2015-07-25 | E-GEOD-71304 | biostudies-arrayexpress

RNA-seq analysis of transcriptomes of WT and Foxp3∆2 Treg cells in spleen and LNs

Project description:We performed RNA-seq analysis of Tregs isolated from Foxp3RFP/∆2Foxp3∆CNS1-cre-Thy1.1Rosa26LSL-YFP female mice. CD4+YFP+RFP+ (WT Tregs) and CD4+YFP+RFP- (Foxp3∆2 Tregs) cells were sorted from lymph nodes and spleen to a typical purity of >95% We detected 638 differentially expressed genes (DEGs, FDR < 0.05) between Foxp3∆2 and WT Tregs

2023-07-01 | GSE208094 | GEO

Transcriptional deregulation in EZH2-deficient lymphocyte progenitors

Project description:This goal of this study was to identify genes that are deregulated in the absence of EZH2 in early lymphocyte progenitors. We examined gene expression by RNA-sequencing in sorted CLPs (Lin-CD117int CD127+ CD135+), pro-B cells (B220+CD19+CD43+), DN3 cells (Lin- CD25+ CD117-), splenic NK cells (Lin-NK1.1+DX5+) and bone marrow ILC2 cells (Lin- Sca1+CD127+) from Ezh2fl/fl Il7racre/+ and Il7racre/+ control mice. Reads were aligned to the mm10 reference genome by Tophat2.1.0. Reads were assigned to genes using the htseq-count tool from HTSeq v 0.6.1 and gene annotations from Ensembl release 78. Differential expression was calculated across 2-3 independent replicates by EdgeR. We found that CLPs, ILC2s, and splenic NK cells maintained their normal transcriptional programs despite loss of EZH2. In contrast, loss of EZH2 caused over 1000 genes to be deregulated in pro-B and DN3 cells indicating that EZH2 is required for transcriptomic stability in adaptive, but not innate lymphocyte progenitors.

2017-04-07 | GSE97462 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data