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Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE189599 | GEO | 2022/06/01

REPOSITORIES: GEO

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Identification and characterization of BEND2 as a key regulator of meiosis during mouse spermatogenesis.

Project description:The chromatin state, which undergoes global changes during spermatogenesis, is critical to meiotic initiation and progression. However, the key regulators involved and the underlying molecular mechanisms remain to be uncovered. Here, we report that mouse BEND2 is specifically expressed in spermatogenic cells around meiotic initiation and that it plays an essential role in meiotic progression. Bend2 gene knockout in male mice arrested meiosis at the transition from zygonema to pachynema, disrupted synapsis and DNA double-strand break repair, and induced nonhomologous chromosomal pairing. BEND2 interacted with chromatin-associated proteins that are components of certain transcription-repressor complexes. BEND2-binding sites were identified in diverse chromatin states and enriched in simple sequence repeats. BEND2 inhibited the expression of genes involved in meiotic initiation and regulated chromatin accessibility and the modification of H3K4me3. Therefore, our study identified BEND2 as a previously unknown key regulator of meiosis, gene expression, and chromatin state during mouse spermatogenesis.

| S-EPMC9132480 | biostudies-literature

Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis [ATAC_seq]

Project description:To describe the changes of chromatin accessibility in Bend2 knockout mice, we conducted ATAC-seq analyses by using zygotene spermatocytes from Bend2 WT and KO mice. We found the chromatin state were opened in the proximal promoter of Bend2 up-regulated genes.

2022-06-01 | GSE189597 | GEO

Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis [Histone_CUTRUN]

Project description:To describe the changes of histone modifications in Bend2 knockout mice, we conducted CUT&RUN analyses by using zygotene spermatocytes from Bend2 WT and KO mice with H3K4me3 and H3K27me3 antibodies. We found the enrichment of these histone modifications in the proximal promoter of Bend2 down-regulated genes were disrupted in Bend2 KO mice.

2022-06-01 | GSE189596 | GEO

Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis [BEND2_ChIPseq]

Project description:To characterize BEND2-binding sites on the genome, we conducted ChIP-seq analyses by using testicular lysates from FLAG-BEND2 KI mice. We found BEND2 preferentially binds to simple sequence repeats.

2022-06-01 | GSE189594 | GEO

Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis [RNA-seq]

Project description:To describe the changes of transcriptome in Bend2 knockout mice, we conducted RNA-seq analyses by using leptotene and zygotene spermatocytes from Bend2 WT and KO mice. We found BEND2 contributes to shutting down the mitotic program and to activating or enhancing the meiotic and post-meiotic program of spermatogenic cells.

2022-06-01 | GSE189598 | GEO

Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis [RNA-Seq II]

Project description:To describe the changes of transcriptome in Bend2 knockout mice, we conducted RNA-seq analyses by using spermatocytes from d12 (12-days after birth) Bend2 WT and KO mice. We found BEND2 contributed to inhibiting the expression of genes involved in spermatogonia differentiation and meiosis initiation.

2022-06-01 | GSE196004 | GEO

Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis

Project description:Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis

| PRJNA783812 | ENA

Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis [ATAC_seq]

Project description:Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis [ATAC_seq]

| PRJNA783813 | ENA

Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis [BEND2_ChIPseq]

Project description:Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis [BEND2_ChIPseq]

| PRJNA783816 | ENA

Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis [Histone_CUTRUN]

Project description:Identification and characterization of BEND2 as a novel and key regulator of meiosis during mouse spermatogenesis [Histone_CUTRUN]

| PRJNA783814 | ENA

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