Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mutation of Vsx genes in zebrafish highlights the robustness of the retinal specification network

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Danio rerio

PROVIDER: GSE189739 | GEO | 2023/05/12

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Mutation of Vsx genes in zebrafish highlights the robustness of the retinal specification network [ATAC-Seq]

Project description:Genetic studies in human and mice have established a dual role for Vsx genes in retina development: an early function in progenitors’ specification, and a later requirement for bipolar-cells fate determination. Despite their conserved expression patterns, it is currently unclear to which extent Vsx functions are also conserved across vertebrates, as mutant models are available only in mammals. To gain insight into vsx function in teleosts, we have generated vsx1 and vsx2 CRISPR-Cas9 double knockouts (vsxKO) in zebrafish. Our electrophysiological and histological analyses indicate severe visual impairment and bipolar cells depletion in vsxKO larvae, with retinal precursors being rerouted towards photoreceptors or Müller glia fates. Surprisingly, neural retina is properly specified and maintained in mutant embryos, which do not display microphthalmia. We show that although important cis-regulatory remodelling occurs in vsxKO retinas during early specification, this has little impact at a transcriptomic level. Our observations point to genetic redundancy as an important mechanism sustaining the integrity of the retinal specification network, and to Vsx genes regulatory weight varying substantially among vertebrate species.

2023-05-12 | GSE189738 | GEO

Mutation of Vsx genes in zebrafish highlights the robustness of the retinal specification network [RNA-Seq]

2023-05-12 | GSE189737 | GEO

Mutation of Vsx genes in zebrafish highlights the robustness of the retinal specification network

Project description:Mutation of Vsx genes in zebrafish highlights the robustness of the retinal specification network

| PRJNA784293 | ENA

Mutation of Vsx genes in zebrafish highlights the robustness of the retinal specification network [RNA-Seq]

Project description:Mutation of Vsx genes in zebrafish highlights the robustness of the retinal specification network [RNA-Seq]

| PRJNA784298 | ENA

Mutation of Vsx genes in zebrafish highlights the robustness of the retinal specification network [ATAC-Seq]

Project description:Mutation of Vsx genes in zebrafish highlights the robustness of the retinal specification network [ATAC-Seq]

| PRJNA784297 | ENA

Epidermal basal domains organization highlights skin robustness to environmental exposure.

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-07-05 | GSE205746 | GEO

Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-09-09 | E-GEOD-54901 | biostudies-arrayexpress

Tbx2b is required for ultraviolet photoreceptor cell specification during zebrafish retinal development.

Project description:The vertebrate rod and cone photoreceptors are highly specialized sensory neurons that transduce light into the chemical and electrical signals of the nervous system. Although the physiological properties of cones and rods are well known, only a handful of genes have been identified that regulate the specification of photoreceptor subtypes. Taking advantage of the mosaic organization of photoreceptors in zebrafish, we report the isolation of a mutation resulting in a unique change in photoreceptor cell fate. Mutation of the lots-of-rods (lor) locus results in a near one-for-one transformation of UV-cone precursors into rods. The transformed cells exhibit morphological characteristics and a gene-expression pattern typical of rods, but differentiate in a temporal and spatial pattern consistent with UV-cone development. In mutant larvae and adults, the highly ordered photoreceptor mosaic is maintained and degeneration is not observed, suggesting that lor functions after the specification of the other photoreceptor subtypes. In genetic chimeras, lor functions cell-autonomously in the specification of photoreceptor cell fate. Linkage analysis and genetic-complementation testing indicate that lor is an allele of tbx2b/fby (from beyond). fby was identified by a pineal complex phenotype, and carries a nonsense mutation in the T-box domain of the tbx2b transcription factor. Homozygous fby mutant larvae and lor/fby transheterozygotes also display the lots-of-rods phenotype. Based upon these data, we propose a previously undescribed function for tbx2b in photoreceptor cell precursors, to promote the UV cone fate by repressing the rod differentiation pathway.

| S-EPMC2632714 | biostudies-literature

Asymmetric robustness in the feedback control of the retinoic acid network response to environmental disturbances

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-09-29 | GSE154408 | GEO

PAX6D instructs neural retinal specification from human embryonic stem cell-derived neuroectoderm

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-07-11 | GSE128141 | GEO