Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profiling of Plasmodium falciparum after co-culture with NK cells

ABSTRACT: The aim of the study was to determine the effect of natural killer (NK) cells on the global gene expression in Plasmodium falciparum.

ORGANISM(S): Plasmodium falciparum Anopheles gambiae

PROVIDER: GSE19010 | GEO | 2012/07/01

SECONDARY ACCESSION(S): PRJNA121073

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression profiling of Plasmodium falciparum after co-culture with NK cells

Project description:The aim of the study was to determine the effect of natural killer (NK) cells on the global gene expression in Plasmodium falciparum. FCR3-CSA-infected red blood cells (RBCs) were co-cultured for 24h with NK92. Prior to RNA extraction, the parasitized RBCs were separated via Ficoll from the lymphocyte fraction. Control cultures of parasites without NK cells were cultivated in parallel. In addition, the effect of the separation method was determined via a Ficoll control culture of FCR3-CSA that was passed over a Ficoll gradient. All three treatments were performed in triplicate. The RNA was reverse-transcribed and hybridized onto microarrays.

2012-06-30 | E-GEOD-19010 | biostudies-arrayexpress

Expression profile of human natural killer cells co-cultured with P. falciparum infected erythrocytes

Project description:In order to identify an expression signature that might characterize the impact of Plasmodium parasites on NK cells, we have used Affymetrix oligonucleotide microarrays to examin the global gene expression profile of primary NK cells that have been co-incubated with P. falciparum infected red blood cells and have compared it to the expression pattern of NK genes induced by cytokine treatment. PBMCs were isolated from 3 healthy donors and were incubated with iRBCs, uRBCs, IL12 plus IL18 and without any stimulus. After 24 hours at 37ºC and 5%CO2, Natural killer cells were isolated by negative selection, checked for purity by flow cytometry and processed for RNA extraction and analysis with the Human Gene 1.0 Affymetrix array. The experiment was repeated 3 times (1-2 weeks apart) for each one of the 3 donors.

2011-12-01 | E-GEOD-24791 | biostudies-arrayexpress