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Gpr125 is a Unifying Hallmark of Multiple Mammary Progenitors coupled with Tumor Latency

ABSTRACT: Several markers have been used to identify mammary stem cell subpopulations. However, a specific unifying hallmark of progenitors has not yet been identified yet. Here we show the orphan adhesion G-protein coupled receptor, Gpr125, identifies and locates long-lived progenitors at multiple sites and stages of mammary development. In particular, Gpr125 is expressed in disparate cell populations with documented regenerative capacity in the nipple-proximal and in the TEB-distal end of the mammary gland. To compare these two major Gpr125 cell populations we used whole RNAseq genome to characterize their gene expression profile. Our data revealed Gpr125+ cells at the nipple-proximal end of the gland are engaged in chemorepulsion and the formation of cell surface structures associated with cell movement. In contrast Gpr125+ cells at the TEB-distal tips exhibit a hybrid epithelial-mesenchymal phenotype and are equipped to bind chemokine and growth factors and secrete a promigratory matrix.

ORGANISM(S): Mus musculus

PROVIDER: GSE190180 | GEO | 2022/01/27

REPOSITORIES: GEO

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