Transcriptomics

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Bulk RNA sequencing of cytotoxic T lymphocytes of OT1 mice


ABSTRACT: We found that MC38 tumor-conditioned medium(TCM) treatment significantly impacted multiple pathways including cholesterol metabolism pathway of CTLs. Further analysis of cholesterol metabolism pathway, we observed that Cholesterol-25-hydroxylase(Ch25h) expression was significantly downregulated after treated with TCM compared to treated with fibroblasted-conditioned medium(FCM). In the meanwhile, we found that activating transcription fator 3(Atf3) was signifcantly upregulated after TCM treatment. Finally, we investigated the Atf3-Ch25h axis regulating CTLs function in tumor context.

ORGANISM(S): Mus musculus

PROVIDER: GSE190702 | GEO | 2023/09/21

REPOSITORIES: GEO

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