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Arabidopsis Col-5 challenged with Ralstonia solanacearum BCCF401

ABSTRACT: Arabidopsis plants were challenged with Ralstonia solanacearum isolate BCCF401 and expression profiles investigated during early and late wilt symptom development. Keywords: Disease state analysis

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE19178 | GEO | 2010/11/22

SECONDARY ACCESSION(S): PRJNA120597

REPOSITORIES: GEO

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Arabidopsis Col-5 challenged with Ralstonia solanacearum BCCF401

Project description:Arabidopsis plants were challenged with Ralstonia solanacearum isolate BCCF401 and expression profiles investigated during early and late wilt symptom development. Keywords: Disease state analysis A direct comparison was performed. Two replicate biological experiments were performed and two technical replicates with dye swap were included in each biological replicate.

2010-11-22 | E-GEOD-19178 | biostudies-arrayexpress

Expression data from Arabidopsis thaliana Col-0 challenged with Ralstonia solanacearum race 4

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Expression data from Arabidopsis thaliana Col-0 challenged with Ralstonia solanacearum race 4 (roots)

Project description:Bacterial wilt caused by Ralstonia solanacearum is a serious seed/soil borne disease that causes severe yield and quality losses in many plants. In order to understand the change in genome expression of inculated plants, microarray analysis were performed. Twenty one days old roots of Arabidopsis Col-0 were inoculated with Ralstonia solanacearum race 4 @ 10^9 & 10^8 cfu/ml in different plants, distil water were mock inoculated, after five days plants were taken for RNA extraction and hybridization on Affymetrix microarrays. Plants were incubated in growth chamber for disease development, temperature and humidity were maintained as per plant requirement for both treated and control plants.

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Project description:Bacterial wilt caused by Ralstonia solanacearum is a lethal, soil-borne disease of tomato. Control of the disease with chemicals and crop rotation is insufficient, because the pathogen is particularly well adapted for surviving in the soil and rhizosphere. Therefore, cultivar resistance is the most effective means for controlling bacterial wilt, but the molecular mechanisms of resistance responses remain unclear. We used microarrays to obtain the characteristics of the gene expression changes that are induced by R. solanacearum infection in resistant cultivar LS-89 and susceptible cultivar Ponderosa.

2012-10-06 | GSE31807 | GEO

Master transcription factors of plant immunity are targeted by a Ralstonia solanacearum effector

Project description:We found the Type III effector protein RipAB could suppress multiple plant immune responses and is important for the virulence of bacterial wilt pathogen Ralstonia solanacearum.

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Response of two clavata 1 mutants to Ralstonia solanacearum.

Project description:In this experiment, we compared transcriptome of 2 homozygous knock out mutants in the CLV1 gene (clv1-12 and clv1-13) to their wild type Ws-2 when challenged with the virulent Rd15 strain of Ralstonia solanacearum. The two mutants show a significant delay of symptom appearance. The experiment was conducted on roots and leaves of 4 week old plants harvested at the time of inoculation and when the first symptoms appeared on the susceptible wild type (4 days after inoculation).

2016-03-25 | E-MTAB-3400 | biostudies-arrayexpress

Response of clavata 2-7 mutant to Ralstonia solanacearum.

Project description:In this experiment, we compared transcriptome of one knock out mutants in the CLV2 gene (clv2-7) to his wild type Col-0 when challenged with the virulent GMI1000 strain of Ralstonia solanacearum. This mutant shows a significant delay of symptom appearance. The experiment was conducted on roots and leaves of 4 week old plants harvested at the time of inoculation and when the first symptoms appeared on the susceptible wild type (4 days after inoculation - 25% of wilted leaves: disease index 1, D1).

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Gene expression analysis of Mulberry root after inoculation with Ralstonia solanacearum.

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2019-06-13 | GSE60030 | GEO

Expression analysis of Ralstonia solanacearum strain GMI1000 at 20°C and 28°C in rich medium (CPG) and in planta

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Expression analysis of Ralstonia solanacearum strain UW551 at 20°C and 28°C in rich medium (CPG) and in planta

Project description:Investigation of whole genome gene expression level changes in the bacterial wilt pathogen Ralstonia solanacearum, strain UW551 at 20°C and 28°C in culture and in planta. The temperatel strain UW551 can wilt and cause full-blown disease on tomato plants at 28°C as well as at 20°C. A 16 array study using total RNA recovered from the following: 8 separate cultures of Ralstonia solanacearum strain UW551 grown in rich medium-CPG (4 grown at 20°C and 4 grown at 28°C) 8 separate in planta samples of Ralstonia solanacearum strain UW551 harvested from diseased tomato plants cv. BonnyBest (4 recovered from plants grown at 20°C and 4 from plants grown at 28°C) Each array (4-plex format) measures the expression level of 4318 genes from Ralstonia solanacearum strain UW551 with 2 to 6, 40-70 mer probes per gene, with two-fold technical redundancy. The arrays also contain probes for intergenic regions with no technical replicates.

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