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Translational profiling of zebrafish retina cells using translating ribosome affinity purification methods

ABSTRACT: Translating ribosome affinity purification (TRAP) methods allow cell-specific recovery of polyribosome-associated RNAs by genetic tagging of ribosomes in selected cell populations. In this study, we purified and analysed the polyribosome-associated RNA fraction of zebrafish embryo´s retinas at 22 hours post-fertilization. To this aim, we generated a transgenic strain in which the tagged ribosomes expression is restricted to the retina cells due to the activity of a specific promoter.

ORGANISM(S): Danio rerio

PROVIDER: GSE192511 | GEO | 2021/12/29

REPOSITORIES: GEO

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