Genomics

Dataset Information

0

ADAR1 controls the miR-381-3p-mediated expression of MRP4 by regulating the production of circHIPK3 in human renal cell


ABSTRACT: Multidrug resistance-associated protein 4 (MRP4), a member of the C subfamily of ATP-binding cassette transporters, is highly expressed in the kidney of mammals and responsible for renal elimination of various drugs. Adenosine deaminase acting on RNA 1 (ADAR1) has been reported to regulate gene expression through catalyzing adenosine-to-inosine (A-to-I) RNA editing. In this study, we found that the down-regulation of ADAR1 increased the expression of MRP4 in human renal cells at post-transcriptional level. The results of luciferase reporter assays and microarray analysis revealed that down-regulation of ADAR1 decreased the levels of miR-381-3p, which led to the up-regulation of MPR4 expression. Circular RNAs (circRNAs) are a kind of closed loop endogenous non-coding RNAs that play a critical role of gene expression by acting as miRNA sponges. ADAR1 repressed the biogenesis of circular RNA circHIPK3 by catalyzing A-to-I RNA editing, which changed the secondary structure of precursor of circHIPK3. In silico analysis suggested that circHIPK3 acted as a sponge of miR-381-3p. Indeed, overexpression of circHIPK3 up-regulated the expression of MRP4 through interfering with miR-381-3p. Our results provide the novel point of view for regulating mechanism of the expression of xenobiotic transporters through mediating the control of circRNA expression by RNA editing enzyme ADAR1.

ORGANISM(S): Homo sapiens

PROVIDER: GSE192692 | GEO | 2022/07/27

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

| PRJNA792611 | ENA
2018-04-08 | GSE67660 | GEO
2017-06-25 | GSE100210 | GEO
2014-12-19 | E-GEOD-62917 | biostudies-arrayexpress
2018-02-09 | GSE99249 | GEO
2015-03-23 | GSE56152 | GEO
2015-12-31 | E-GEOD-67496 | biostudies-arrayexpress
2012-08-31 | E-GEOD-31963 | biostudies-arrayexpress
2016-09-05 | E-GEOD-60217 | biostudies-arrayexpress
2022-08-24 | PXD024974 | Pride