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DNA methylation profiling in lungs of Th17-mediated neutrophil-predominant asthma mice vs control mice

ABSTRACT: Recently, epigenetic regulation was shown to play an important role in asthma development. In particular, DNA methylation, as one of the important epigenetic modifications, has been proved to be related to the pathogenesis of asthma. However, little is known about the DNA methylation status in Th17-mediated neutrophil-predominant asthma. To investigate the potential functions of DNA methylation that affect the pathogenesis of Th17-mediated neutrophil-predominant asthma, a methylated DNA immunoprecipitation (MeDIP) chip was used to detect the whole genome single-base DNA methylation profiles of lungs from Th17-mediated neutrophil-predominant asthma mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE192781 | GEO | 2024/12/29

REPOSITORIES: GEO

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Project description:Background: MicroRNAs are potent regulators of biologic systems that are critical to tissue homeostasis. Individual microRNAs have been identified in airway samples. However, a systems analysis of the microRNA-mRNA networks present in the sputum that contribute to airway inflammation in asthma has not been published. Methods: We conducted a genome-wide analysis of microRNA and messenger RNA (mRNA) in the sputum from patients with asthma and correlated expression with clinical phenotypes. Weighted gene correlation network analysis (WGCNA) was implemented to identify microRNA networks (modules) that significantly correlate with clinical features of asthma and mRNA expression networks. MicroRNA expression in peripheral blood neutrophils and lymphocytes, and in situ hybridization of the sputum were used to identify the cellular sources of microRNAs. MicroRNA expression obtained before and after ozone exposure was also used to identify changes associated with neutrophil counts in the airway. Results: Six microRNA modules were associated with clinical features of asthma. A single module (nely) was associated with a history of hospitalizations, lung function impairment, and numbers of neutrophils and lymphocytes in the sputum. Of the 12 microRNAs in the nely module, hsa-miR-223-3p was the highest expressed microRNA in neutrophils and was associated with increased neutrophil counts in the sputum in response to ozone exposure. Multiple microRNAs in the nely module correlated with two mRNA modules enriched for toll-like receptor (TLR) and Th17 signaling, and endoplasmic reticulum stress. Hsa-miR-223-3p was a key regulator of the TLR and Th17 pathways in the sputum of subjects with asthma. Conclusions: This study of sputum microRNA and mRNA expression from patients with asthma demonstrates the existence of microRNA networks and genes that are associated with features of asthma severity. Among these, hsa-miR-223-3p, a neutrophil-derived microRNA, regulates TLR/Th17 signaling and endoplasmic reticulum stress.

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DNA methylation profiling in lungs of Th17-mediated neutrophil-predominant asthma mice vs control mice

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