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CroR-regulated genes in Enterococcus faecalis OG1

ABSTRACT: The goal was to identify genes that are regulated by cell wall stress (bacitracin treatment) in wild-type cells but not in mutant cells lacking CroR, thereby revealing genes of the CroR regulon. Cells of a mutant lacking CroR or its otherwise isogenic wild-type E. faecalis OG1 were cultured in Mueller-Hinton broth and treated (or not) with bacitracin for 15 min. Total RNA was purified, depleted for rRNA, and subjected to preparation of TruSeq stranded mRNA libraries before sequencing on a NextSeq 550 (76-bp single read run).

ORGANISM(S): Enterococcus faecalis

PROVIDER: GSE193042 | GEO | 2022/07/25

REPOSITORIES: GEO

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