The profiles of m6A modification in villous tissues with embryo damage

ABSTRACT: A growing number of studies have demonstrated that N6 methyladenine (m6A) acts as an important role in the pathogenesis of reproductive diseases, which makes it essential to profile the genome-wide m6A modifications in embryo damage. In this study, due to the microscopicity of early villous tissue, we performed high-throughput sequencing for villous tissues from three patients with embryo damage and three patients with painless abortion. Based on meRIP-seq data, we identified 18,568 m6A peaks from these two samples. These m6A peaks were mainly located in the coding region near the stop codon and were mainly characterized by AUGGAC and UGGACG motif. Compared with the normal group, the embryo damage group had 2,159 significantly upregulated m6A peaks and 281 downregulated m6A peaks. Genes with altered m6A peaks were mainly involved in Hippo and Wnt signaling pathways. Based on RNA-seq data, we found that differentially expressed genes were mainly involved in Th17 cell differentiation, TNF signaling pathway, and ECM-receptor interaction. Based on the conjoint analysis of meRIP-seq and RNA-seq data, we identified 36 genes with differentially methylated m6A peaks and synchronously differential expression. These genes were mainly involved in the Wnt signaling pathway, phosphatase activity regulation, protein phosphatase inhibitor activity, and transcription inhibitor activity. In general, our study revealed the transcriptome-wide m6A methylome in early embryonic development, which may provide novel insights into the pathogenesis and treatment of embryo damage.

ORGANISM(S): Homo sapiens

PROVIDER: GSE193052 | GEO | 2023/08/06

REPOSITORIES: GEO