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RNAseq data of differentiated vs undifferentiated tonsil-derived mesenchymal stem cells into parathyroid-like cells seeded at 100% confluency

ABSTRACT: High-cell density/confluency leads to contact inhibition of proliferation, as seen in the majority of epithelial cells, and is associated with the initiation of differentiation. To determine potential effect of In this regard, we analyzed transcriptomes using RNAseq, then performed a large-scale expression profiling of T-MSCs and performed a comparison between un-differentiated T-MSCs and T-MSC-PTHCs. Genes with a mean fold change of > 1.5 (T-MSCs vs. T-MSC-PTHCs) with p < 0.05 were analyzed using gene ontology enrichment analysis.RNAseq analysis showed that total of 228 genes were found to be significantly changed. Of them, the genes involved in the proliferation contributed 28% (total count of 63 genes) was the largest of the altered gene population followed by the genes involved in neurogenesis (30 counts; 13%) and extracellular matrix (16 counts; 7%). Among the genes in differentiation, WNT2 showed the highest fold-increase (2.8-fold) in the T-MSC-PTHCs compared to un-differentiated T-MSCs. When KEGG pathway analyses were performed, we found WNT signaling and stem cell pluripotency-regulating pathways as the pathways, where the WNT2 was involved in. This indicates that the differentiation of the T-MSCs could have induced through both of these pathways.

ORGANISM(S): Homo sapiens

PROVIDER: GSE193108 | GEO | 2022/01/10

REPOSITORIES: GEO

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