Differential expression of genes involved in salt-treated iU1 seedlings
Ontology highlight
ABSTRACT:
ORGANISM(S): Arabidopsis thaliana
PROVIDER: GSE193841 | GEO | 2022/06/29
REPOSITORIES: GEO
Similar Datasets
Project description:Differential expression of genes involved in salt-treated iU1 seedlings
| PRJNA798070 | ENA
Project description:Purpose: The goals of this study are to compare differentially expressed transcripts in seedlings of watermelon during salt stress using transcriptome profiling (RNA-seq)
2020-08-11 | GSE146087 | GEO
Project description:To gain insight into how AtCAPE1 regulates salt response, we investigated the gene expression profiles of wild-type (Ler) and proatcape1 mutant seedlings in the presence and absence of 125 mM NaCl by microarray analysis.
2015-07-01 | GSE66946 | GEO
Project description:The Fusarium incarnatum strain K23, originally isolated from a habit-adapted wild plant Thapsia species, colonized the roots and shoots of tomato seedlings and protected them against salt stress. Comparison of expression and metabolite profile changes uncovered that the fungus completely reprogramed the tomato response to salt stress. Barely any overlap was observed among the genes and metabolites which are regulated by salt stress in uncolonized and colonized tomato seedlings. In colonized seedlings exposed to salt stress, less stress- related genes are activated than in un-colonized seedlings. Furthermore, K23 produced gibberellin and gibberellin-responsive genes were detected in all RNA samples. Our analysis demonstrates that K23 colonisation completely alters the salt-responsive gene and metabolite profiles in tomato seedlings.
2024-11-30 | GSE255960 | GEO
Project description:Large-scale gene expression affected by salt stress was analyzed with tomato seedlings (Lycoperson esculentum Mill cv. Money Maker) by a cDNA microarray (Tom1). The significantly differentially expressed genes (5% Benjamini-Hochberg false discovery rate) consisted of 1757 sequences in the analyzed tissues (cotyledons + shoot tip). Genes with over 2 fold difference were selected from the list and further categorized into different function and cellular processes. Tomato homologous genes for the chaperone proteins, antioxidant enzymes (catalase and peroxidase), and ion transporters (Na+-driven multidrug efflux pump, vacuolar ATPase, and others) were induced. The ACC oxidase and ethylene-responsive gene tomato homologs had higher transcript level after salt treatment. Multiple members with different expression patterns were identified for the bZIP, WRKY, and MADS-box transcription regulator. Different genes in the signal transduction pathway, such as the protein kinases (Shaggy kinase, mitogen-activated protein kinase, ethylene receptor neverripe, and others), protein phosphatases, calmodulin, G-protein, and the N- myristoyltransferase, were regulated by salt stress. Most of the protease and the inhibitor homologs were suppressed by salt stress. In addition, different isoforms of cytochrome P450, genes for polyamine biosynthesis (putrescine and proline) and detoxification compounds (glutathione and thioredoxin), several key enzyme genes in the metabolic pathways of carbohydrates, amino acids, and fatty acids, were also affected by salt treatment. This study has provided a set of candidate genes, especially those in the regulatory machinery that can be further investigated to define salt stress in tomato and other plant species. Keywords: treatment response
2007-09-05 | GSE8883 | GEO
Project description:To gain insight into how AtCAPE1 regulates salt response, we investigated the gene expression profiles of wild-type (Ler) and proatcape1 mutant seedlings in the presence and absence of 125 mM NaCl by microarray analysis. Ten seedlings were grown vertically on the mesh attached on 1/2 MS medium for 10 days. Seedlings with the mesh were transferred to a new petri dish and then covered by buffer-saturated filter papers with 1/2 MS liquid medium (Control) or with 125 mM NaCl (Salt). Salt-treated seedlings (n=10 for each treatment) were sampled after 12 h. Three independent experiments were performed for the microarray analysis.
2015-07-01 | E-GEOD-66946 | biostudies-arrayexpress
Project description:Large-scale gene expression affected by salt stress was analyzed with tomato seedlings (Lycoperson esculentum Mill cv. Money Maker) by a cDNA microarray (Tom1). The significantly differentially expressed genes (5% Benjamini-Hochberg false discovery rate) consisted of 1757 sequences in the analyzed tissues (cotyledons + shoot tip). Genes with over 2 fold difference were selected from the list and further categorized into different function and cellular processes. Tomato homologous genes for the chaperone proteins, antioxidant enzymes (catalase and peroxidase), and ion transporters (Na+-driven multidrug efflux pump, vacuolar ATPase, and others) were induced. The ACC oxidase and ethylene-responsive gene tomato homologs had higher transcript level after salt treatment. Multiple members with different expression patterns were identified for the bZIP, WRKY, and MADS-box transcription regulator. Different genes in the signal transduction pathway, such as the protein kinases (Shaggy kinase, mitogen-activated protein kinase, ethylene receptor neverripe, and others), protein phosphatases, calmodulin, G-protein, and the N- myristoyltransferase, were regulated by salt stress. Most of the protease and the inhibitor homologs were suppressed by salt stress. In addition, different isoforms of cytochrome P450, genes for polyamine biosynthesis (putrescine and proline) and detoxification compounds (glutathione and thioredoxin), several key enzyme genes in the metabolic pathways of carbohydrates, amino acids, and fatty acids, were also affected by salt treatment. This study has provided a set of candidate genes, especially those in the regulatory machinery that can be further investigated to define salt stress in tomato and other plant species. Keywords: treatment response Effect of 75mM NaCl on gene’s expression in tomato seedlings, Lycoperscon esculentum Mill. cv. Money Maker after 17 d of treatment. Each sample represents a pool of same tissue 20 seedlings grown in two flasks. Experiment was done in triplicate.
2007-09-04 | E-GEOD-8883 | biostudies-arrayexpress
Project description:Soil salinity is one of the major factors that limits area of cultivable land and yield potential of crops. Considered as a moderately salt sensitive economically-important crop, biological processes involved in salt stress response in peanut remain elusive. Publishing of the genomic sequence of cultivar peanut represent a new opportunity for further research. ABA INSENSITIVE 4 (ABI4) is an evolutionary conserved transcription factor. Mutation of ABI4 in Arabidopsis enhanced salt tolerance of seedlings significantly by targeting and down-regulating of HKT1;1. However, few achievements were reported in other aspects. In this study, AhABI4s in peanut were first cloned and silenced via virus-induced gene silencing method. Phenotype analysis showed that down-regulation of AhABI4s enhanced salt tolerance of peanut significantly. According to proteome and phosphoproteome analysis, expression of 1,900 proteins and 2,620 phosphorylation sites were predicted to be affected by silencing of AhABI4s under salt stress. Proteins with ion transporting activity were enriched by GO analysis. Further analysis showed that, 31 proteins may participate in homeostasis maintain of Na+, K+, Ca2+, H+ and Cl- in plant cells. Combined transcriptome and proteome analysis indicated that 63 genes may regulated by AhABI4s directly and 7 of them, ABI5-like5, RABA1f, SCAMP3, PK, RP-S26e, HSP70, cysteine proteinase inhibitor, were able to interact with the predicted ion transporters. Silencing of AhABI4s altered down-stream protein-protein interaction network to regulate expression/phosphorylation level of ion transporters, and finally influence homeostasis under salt stress. This work provides novel insights for salt response mechanism research and offer important evidence for protein function study.
2021-07-18 | PXD015090 | Pride
Project description:A tandem mass tag (TMT)-based comparative peptidomics analysis of rice seedlings under salt stress was conducted. Rice seedlings were exposed to 50 and 150 mM NaCl for 24 and 72 h, respectively, and the root and shoot tissues of different treatment groups were collected separately for the peptidomic analysis.
2023-03-11 | PXD037574 | Pride
Project description:Background Salinization seriously threatens land use efficiency and crop yields across the world. Understanding the mechanisms plants use to protect against salt stress will help breeders develop salt-tolerant vegetable crops. Okra (Abelmoschus esculentus L) is an important vegetable crop of the mallow family, which is now cultivated in warm regions worldwide. To understand the effects of salt stress on the protein level of okra, a comparative proteomic analysis of okra seedlings grown in the presence of 0 or 300 mmol L-1 NaCl treatment was performed using an integrated approach of Tandem Mass Tag labeling and LC-MS/MS integrated approach. Results A total of 7179 proteins was identified in this study, for which quantitative information was available for 5,774 proteins. In the NaCl/control comparison group, there were 317 differentially expressed proteins (DEPs), of which 165 proteins were upregulated and 152 proteins downregulatedin the presence of NaCl. Based on the above data, we carried out a systematic bioinformatics analysis of proteins with information, including protein annotation, domain characteristics,functional classification,and pathway enrichment. Enriched gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the DEPs were most strongly associated with“response to stress” and “protein processing in endoplasmic reticulum”. Furthermore, several heat shock proteins were identified as DEPs. Conclusions This informationprovides a reference direction for further research on the okraproteome in the downstreamof the salt stress response, with our data revealing that the responses of okra to salt stress involves by various pathways.
2019-11-12 | PXD011612 | Pride