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Expression profiles of mRNAs in the retinas of OIR wildtype, OIR IL-19 knockout and room air control mice.

ABSTRACT: Seven-day-old C57BL/6 mice and IL-19 knockout (KO) mice were subjected to 75% oxygen for 5 days to induce OIR. To explore the mechanism that caused by IL-19 on retinal neovascularization, Gene expressions among retinas of room air control mice, OIR wildtype mice, and OIR IL-19 knockout mice were measured by RNA-sequencing (RNA-seq).

ORGANISM(S): Mus musculus

PROVIDER: GSE194176 | GEO | 2022/01/24

REPOSITORIES: GEO

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Expression profiles of mRNAs in the retinas of OIR wildtype, OIR IL-19 knockout and room air control mice.

Project description:Expression profiles of mRNAs in the retinas of OIR wildtype, OIR IL-19 knockout and room air control mice.

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RNA sequencing of sorted P17 murine endothelial cells from the OIR model with OSM or PBS intravitreal injection at P12

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Effects of Aflibercept administration at 25 mg per kg body weight in retinas of mice subjected to the oxygen-induced retinopathy protocol [P50]

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RNA-sequencing of the oxygen induced retinopathy (OIR) model in C57BL/6 mice

Project description:Pathological retinal angiogenesis is one of the major causes of vision loss worldwide. The breakdown of blood vessels and aberrant vessels growth usually lead to hemorrhage, macular edema, fibrotic scar, and retinal detachment. OIR model serves as a proxy for human pathological retinal neovascularization such as proliferative diabetic retinopathy, retinal vein occlusion and retinopathy of prematurity. C57BL/6 mice were exposed to 75% O2 in a hyperoxic chamber from postnatal day P7 to P12 and then returned to room air. The greatest neovascular response occurred at postnatal 17 days (P17) when the mice are put back to room air condition for 5 days. In order to reveal potential genes that involve in pathological neovascularization, we performed transcriptomic analyses of retina of P17.

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2012-12-05 | GSE38762 | GEO

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