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Lariat mapping of DBR1 KO and DBR1 KO+DBR1 addback cells

ABSTRACT: We constructed a DBR1 knockout cell line (C22) using CRISPR in HEK293T cells. Through mapping of lariat reads, lariat levels in the DBR1 - samples are shown to increase dramatically (~20x) relative to wild type cells. Over 60% of this increase in lariat levels is abrogated upon rescue of DBR1 - cells with a DBR1 expression vector

ORGANISM(S): Homo sapiens

PROVIDER: GSE195469 | GEO | 2022/01/29

REPOSITORIES: GEO

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Lariat mapping of a DBR1 CRISPR KO cell line

Project description:We used CRISPR in HEK293T cells to create two DBR1 knockout cell lines (C19 and C22). After high-throughput sequencing of total RNA extracted from these cells, we performed lariat read mapping using the method described in "Large-scale mapping of branchpoints in human pre-mRNA transcripts in vivo" (Taggart et al, 2012). We found lariats in the two DBR1- cell lines to be ~20-fold enriched relative to the levels observed in the HEK293T control samples.

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Lariat mapping of DBR1 KO and DBR1 KO+DBR1 addback cells

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