Transcriptomics

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CCT2 promotes the proliferation and migration of head and neck squamous cell carcinoma via activating PI3K/AKT pathway


ABSTRACT: Purpose: RNA-seq was used to evaluate the effect of CCT2 knockdown in HNSCC cell lines. Methods: CCT2 was knocked down in SAS cell lines through shRNA and then RNA was extracted from control cells and knockdown cells. Results: Using an optimized data analysis workflow, we identified 205 differentially expressed genes (DEG), of which 141 genes were down-regulated and 64 genes up-regulated, respectively (fold change >2 and adjust p-value < 0.05). RT-qPCR was used to confirm the reliability of RNA-seq data. KEGG and GSEA analysis indicated that PI3K/AKT signaling pathway might be involved in the function of CCT2, which had been proved by western blotting.

ORGANISM(S): Homo sapiens

PROVIDER: GSE195850 | GEO | 2022/02/03

REPOSITORIES: GEO

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