PCBP-1 regulates the transcription and alternative splicing of inflammation and ubiquitination-related genes in Parkinson’s disease model
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ABSTRACT: In this study, we cloned and constitutively overexpressed PCBP-1 in rat PC12 cells. RNA-seq was performed to analyze PCBP-1-regulated differentially expressed genes (DEGs) and alternative splicing events (ASEs) between control and PCBP1-overexpressed cells. GO and KEGG pathway analyses were performed to identify functional DEGs and alternatively spliced genes. Consequently, we validated PCBP-1-regulated genes using RT-qPCR.Overexpression of PCBP-1 widely regulated the ASE and expression of genes enriched in neuroinflammation and protein ubiquitination, which were also associated with PD pathogenesis. Moreover, RT-qPCR assay verified the PCBP-1-modulated expression of neuroinflammatory genes, like LCN-2, and alternative splicing (AS) of ubiquitination-related gene WWP-2
ORGANISM(S): Rattus norvegicus
PROVIDER: GSE196153 | GEO | 2022/07/13
REPOSITORIES: GEO
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