Project description:Genetically engineered mouse models (GEMM) of cancer are powerful tools to study multiple aspects of caner biology. We developed a novel GEMM for lung squamous cell carcinoma (LSCC) by genetically combining overexpression of Sox2 with loss of Lkb1: Rosa26LSL-Sox2-IRES-GFP;Lkb1fl/fl (SL). We compared gene expression profiles of SL lung tumors with normal mouse lung tissue, mouse lung adenocarcinoma (LADC) tumors from KrasLSL-G12D/+;Trp53fl/fl (KP), mouse LSCC tumors from Lkb1fl/fl;Ptenfl/fl (LP) model as well as Lenti-Sox2-Cre Lkb1fl/fl.
Project description:We report the expression profiling of lung adenocarcinoma tumors arising from genetically engineered mice with activation of KRAS(K) and loss of either SMARCA4 (S), p53(P) or both.
Project description:Genome wide DNA methylation profiling of rhabdomuyosarcoma tumors derived from genetically engineered mouse models. The Illumina MouseMethylation285 BeadChip was used to obtain DNA methylation profiles across approximately 285,000 methylation sites in 31 freshly-frozen rhabdomyosarcoma tumors.
