RNA profiling in lungs of WT, IFN-g-/- and IL-4-/- mice before and after Mycoplasma pulmonis infection
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ABSTRACT: Full title: Mouse Inflammatory Cytokines and Receptors Microarray-RNA profiling in lungs of WT, IFN-g-/- and IL-4-/- mice before and after Mycoplasma pulmonis infection RNA profiling of inflammatory chemokines, cytokines and receptors in lungs of 4-6 week old wild-type, IFN-g-/- and IL-4-/- BALB/cJ mice before and after 14-day Mycoplasma pulmonis infection
Project description:Full title: Mouse Inflammatory Cytokines and Receptors Microarray-RNA profiling in lungs of WT, IFN-g-/- and IL-4-/- mice before and after Mycoplasma pulmonis infection RNA profiling of inflammatory chemokines, cytokines and receptors in lungs of 4-6 week old wild-type, IFN-g-/- and IL-4-/- BALB/cJ mice before and after 14-day Mycoplasma pulmonis infection RNA pooled from lungs of each group- Uninfected and Infected; Experiment performed twice. With 3 mice in each of the uninfected and infected groups in the first experiment and 4 mice in each group in the second experiment; comparison Mycoplasma infected vs. uninfected mice within each strain of mice
Project description:RNA profiling of inflammatory chemokines, cytokines and receptors in AUTOMACS sorted F4/80+ and CD11c+ cells from lungs of 6-12 week old wild-type C3H/HeN mice before and after 14-day Mycoplasma pulmonis infection
Project description:RNA profiling of inflammatory chemokines, cytokines and receptors in AUTOMACS sorted F4/80+ and CD11c+ cells from lungs of 6-12 week old wild-type C3H/HeN mice before and after 14-day Mycoplasma pulmonis infection RNA pooled from pulmonary F4/80+ & CD11c+ cells of each group- Uninfected and Infected; Experiment performed twice. Comparison-RNA expression within each population of cells in Mycoplasma infected vs. uninfected mice
Project description:The study aims at understanding the global nature of the immune responses within the the lung tissue 28 days post infection with Mycobacterium tuberculosis. Comparing the whole transcriptome of infected lungs to that of an uninfected lungs revealed a plethora of immune mechanisms driven by various cytokines. IFN-γ, IL-6, IL-2, TNFα were the major cytokines observed. We observed significant differential expression of gene involved in the JAK-STAT and the MAPK signalling pathways. We observed an interplay of the immune regulatory genes and various non-immune genes controlling the metabolism, apoptosis, cell growth, post translational modifications etc.
Project description:Analysis of H292 cells infected with Mycoplasma hyorhinis. Mycoplasma infection reduces the cytotoxic effect of Nutlin3 on H292 cells. The results provide insight into molecular mechanisms underlying the response of H292 cells to Nutlin3.
Project description:Mycoplasma hyopneumoniae, the causative agent of swine enzootic pneumonia, colonizes the cilia of swine lungs, causing ciliostasis and cell death. Mycoplasma hyopneumoniae is a component of the porcine respiratory disease complex (PRDC) and is especially problematic for the finishing swine industry, causing the loss of hundreds of millions of dollars in farm revenues worldwide. For successful infection, M. hyopneumoniae must effectively resist oxidative stresses due to the release of oxidative compounds from neutrophils and macrophages during the host’s immune response. However, the mechanism M. hyopneumoniae uses to avert the host response is still unclear. To gain a better understanding of the transcriptional responses of M. hyopneumoniae under oxidative stress, cultures were grown to early exponential phase and exposed to 0.5% percent hydrogen peroxide for 15 minutes. RNA samples from these cultures were collected and compared to RNA samples from control cultures using two-color PCR-based M. hyopneumoniae microarrays. This study revealed significant down-regulation of important glycolytic pathway genes and gene transcription proteins, as well as a protein known to activate oxidative stressor cascades in neutrophils. This study has also contained significantly differentially expressed genes common to other environmental stress responses, and merits further study of universal stress response genes of M. hyopneumoniae. Keywords: Mycoplasma hyopneumoniae, RNA microarray
Project description:DCs are localized under the mucosa of the lungs and the gastrointestinal tract, and therefore come into close contact with A. fumigatus germ tubes during early steps of infection as soon as fungi become invasive. For a more detailed insight into differentially regulated genes, whole genome microarray analysis was performed. On average, unstimulated DCs showed expression of about 13.500 genes (35% of all genes spotted on the chip). After 6h co-cultivation of DCs and live A. fumigatus germ tubes, 590 genes showed a 4fold altered gene expression, if normalizing data with VSN before. Therein, a wide range of immune response genes, including genes encoding for cytokines (IL-1α, IL-1β, IL-6, IL-8, IL-10, IL-12p40, TNF-α), chemokines (CCL5, CCL20, CXCL10), immunorelevant receptors (TLR2, TLR4, PTX-3), as well as costimulatory molecules (CD40, CD80, CD83, CD86) were differentially regulated. Keywords: dendritic cell, A. fumigatus germ tubes, infection for 6h, affymetrix whole genome microarray analysis
Project description:DCs are localized under the mucosa of the lungs and the gastrointestinal tract, and therefore come into close contact with A. fumigatus germ tubes during early steps of infection as soon as fungi become invasive. For a more detailed insight into differentially regulated genes, whole genome microarray analysis was performed. On average, unstimulated DCs showed expression of about 13.500 genes (35% of all genes spotted on the chip). After 6h co-cultivation of DCs and live A. fumigatus germ tubes, 590 genes showed a 4fold altered gene expression, if normalizing data with VSN before. Therein, a wide range of immune response genes, including genes encoding for cytokines (IL-1α, IL-1β, IL-6, IL-8, IL-10, IL-12p40, TNF-α), chemokines (CCL5, CCL20, CXCL10), immunorelevant receptors (TLR2, TLR4, PTX-3), as well as costimulatory molecules (CD40, CD80, CD83, CD86) were differentially regulated. Experiment Overall Design: Human DCs were generated from two independent, healthy blood donors. 5 x 106 DCs were either cultivated without fungi or together with 5 x 106 A. fumigatus germ tubes for 6h until isolation of total RNA.
Project description:Mouse Inflammatory Cytokines and Receptors Microarray-RNA profiling in F4/80+ and CD11c+ cells from lungs of WT mice before and after Mycoplasma pulmonis infection