Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genetic Analysis of Variation in Transcription Factor Binding in Yeast

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE19636 | GEO | 2010/02/18

SECONDARY ACCESSION(S): PRJNA122523

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

ChIP-exo analysis of the DNA-binding sites of the yeast transcription factor Yfl052w

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-07-22 | E-GEOD-57902 | biostudies-arrayexpress

Fucntional genetic variants mediate their regulatory effects through altered transcription factor binding.

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-06-04 | GSE117576 | GEO

Genetic analysis of variation in transcription factor binding in yeast.

Project description:Variation in transcriptional regulation is thought to be a major cause of phenotypic diversity. Although widespread differences in gene expression among individuals of a species have been observed, studies to examine the variability of transcription factor binding on a global scale have not been performed, and thus the extent and underlying genetic basis of transcription factor binding diversity is unknown. By mapping differences in transcription factor binding among individuals, here we present the genetic basis of such variation on a genome-wide scale. Whole-genome Ste12-binding profiles were determined using chromatin immunoprecipitation coupled with DNA sequencing in pheromone-treated cells of 43 segregants of a cross between two highly diverged yeast strains and their parental lines. We identified extensive Ste12-binding variation among individuals, and mapped underlying cis- and trans-acting loci responsible for such variation. We showed that most transcription factor binding variation is cis-linked, and that many variations are associated with polymorphisms residing in the binding motifs of Ste12 as well as those of several proposed Ste12 cofactors. We also identified two trans-factors, AMN1 and FLO8, that modulate Ste12 binding to promoters of more than ten genes under alpha-factor treatment. Neither of these two genes was previously known to regulate Ste12, and we suggest that they may be mediators of gene activity and phenotypic diversity. Ste12 binding strongly correlates with gene expression for more than 200 genes, indicating that binding variation is functional. Many of the variable-bound genes are involved in cell wall organization and biogenesis. Overall, these studies identified genetic regulators of molecular diversity among individuals and provide new insights into mechanisms of gene regulation.

| S-EPMC2941147 | biostudies-literature

ChIP-exo analysis of the DNA-binding sites of the yeast transcription factor Yfl052w

Project description:This SuperSeries is composed of the SubSeries listed below.

2014-07-22 | GSE57902 | GEO

Nucleosome driven transcription factor binding and gene regulati

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2012-12-01 | E-GEOD-41617 | biostudies-arrayexpress

Genetic Variation Determines PPARγ Function and Antidiabetic Drug Response In Vivo

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-07-02 | E-GEOD-64460 | biostudies-arrayexpress

Genetic Variation, Not Cell Type of Origin, Underlies Regulatory Differences in iPSCs

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-01-19 | E-GEOD-65079 | biostudies-arrayexpress

Genetic determinants and epigenetic effects of pioneer factor binding

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-01-29 | GSE90456 | GEO

Genetic, functional and molecular features of glucocorticoid receptor binding

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-04-01 | E-GEOD-45640 | biostudies-arrayexpress

Conserved and diverged functions of the calcineurin-activated Prz1 transcription factor in fission yeast

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-02-10 | E-GEOD-77761 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data