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Transcriptomic changes in Sin3A deficient CD4 T cells

ABSTRACT: Highly purified CD4+ T cells from Rosa26-CreERT2 +/- Sin3AF/F micewere then cultured in interleukin 7 (IL-7) in the absence or the presence of 4-hydroxytamoxifen (TAM) for 6 days to allow Cre expression in the absence of cell division or differentiation . Next, we compared the behavior of Sin3A sufficient and deficient T cells in Th17 skewing conditions. RNA was then isolated and sequenced for the analysis of differential expression of mRNA transcripts

ORGANISM(S): Mus musculus

PROVIDER: GSE196615 | GEO | 2023/03/20

REPOSITORIES: GEO

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