Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing of HUVECs stimulated with VEGF, OSM+VEGF, or CNTF+CNTFR+VEGF

ABSTRACT: IL-6 family cytokines as OSM and CNTF modify angiogenesis to different degree. This study investigates to which extend treatment of endothelial cells with those cytokines can modify VEGF induced angiogenic reactions. Human umbilical vein endothelial cells (HUVECs) were stimulated for 24h by cytokines followed by RNA harvesting and processed for RNA sequencing.

ORGANISM(S): Homo sapiens

PROVIDER: GSE196776 | GEO | 2022/12/04

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNA sequencing of HUVECs stimulated with VEGF, OSM+VEGF, or CNTF+CNTFR+VEGF with or without STAT3 knock-down

Project description:The role of STAT3 signaling induced by stimulation of IL-6 cytokine family members such as OSM and CNTF on the angiogenic response of endothelial cells is still not fully understood. To assess the impact of this pathway on angiogenesis, this study compares human umbilical vein endothelial cells (HUVECs) with or without STAT3 knock-down introduced by siRNA and stimulation with VEGF, OSM+VEGF and CNTF+VEGF in the presence of the CNTF receptor (CNTFR).

2022-12-04 | GSE198484 | GEO

RNA sequencing of HUVECs stimulated with VEGF, OSM+VEGF, or CNTF+CNTFR+VEGF

Project description:RNA sequencing of HUVECs stimulated with VEGF, OSM+VEGF, or CNTF+CNTFR+VEGF

| PRJNA807293 | ENA

RNA sequencing of HUVECs stimulated with VEGF, OSM+VEGF, or CNTF+CNTFR+VEGF with or without STAT3 knock-down

Project description:RNA sequencing of HUVECs stimulated with VEGF, OSM+VEGF, or CNTF+CNTFR+VEGF with or without STAT3 knock-down

| PRJNA815366 | ENA

RNA sequencing of sorted P17 murine endothelial cells from the OIR model with OSM or PBS intravitreal injection at P12

Project description:IL-6 family cytokines as OSM modify angiogenesis to different degree. This study investigates the influence of intravitreal injection of OSM on the formation of neovascularization in the mice model of Oxygen-induced retinopathy (OIR). Mice at P12 were injected OSM or PBS , retinal cells isolated at P17 via sort and the transcriptome analyzed by RNA sequencing.

2024-01-09 | GSE227350 | GEO

Transcription profiling of human HUVECs treated with the angiogenic factors VEGF-A and PlGF in low or high serum media

Project description:HUVECs (human umbilical cord vein endothelial cells) are treated with the angiogenic factors VEGF-A (vascular endothelial growth factor-A) and PlGF (placental growth factor) in low or high serum media.

2007-09-27 | E-GEOD-837 | biostudies-arrayexpress

ZIKV infection increases HUVECs endothelial barrier permeability and activates inflammatory cytokines

Project description:ZIKV infection increases HUVECs endothelial barrier permeability and activates inflammatory cytokines

| PRJNA488555 | ENA

Bulk RNAseq of HUVECs

Project description:Bulk RNAseq was performed using P4 human umbilical vein endothelial cells (HUVECs)

2022-07-30 | GSE209855 | GEO

SNAI1 knockdown under VEGF stimulus in HUVECs

Project description:The goal of this study was to reveal SNAI1 function in angiogenesis. SNAI1 is up-regulated by VEGF stimulus. Using RNASeq method (single-ended 150-bp sequencing on Illumina Hi-seq 3000 instrument), we measured the transcriptional difference between siSNAI1 or NC-transfected HUVECs.

2019-03-04 | GSE111386 | GEO

RNA-seq of human umbilical vein endothelial cells (HUVECs) stimulated with recombinant Vascular Endothelial Growth Factor A (VEGF-A) against untreated control

Project description:In order to identify new genes potentially involved in angiogenesis process, we performed a deep-sequencing transcriptome analysis of HUVECs stimulated with recombinant VEGF-A, introducing a difference with respect to the several previous studies. Primary ECs are normally cultured in vitro using an endothelial growth medium (EGM) constituted by an endothelial basal medium (EBM) supplemented with 2% fetal bovine serum (FBS) and a mix of growth factors and reagents, typically represented by basic Fibroblast Growth Factor (bFGF), Insulin-like Growth Factor 1 (IGF1), Epidermal Growth Factor (EGF), heparin, hydrocortisone, ascorbic acid and the same VEGF-A. In previous studies, HUVECs or other primary ECs were starved in EBM with low FBS concentration before the stimulation with VEGF-A. Instead of starvation, we grown HUVECs in complete EGM-2 medium except for VEGF-A to maintain the best condition for in vitro survival and propagation of primary ECs. After 24 hours of VEGF-A deprivation, VEGF-A was re-added to the medium and the stimulation was performed for six hours.

2021-04-08 | E-MTAB-9337 | biostudies-arrayexpress

SNAI1 knockdown under VEGF stimulus in HUVECs

Project description:SNAI1 knockdown under VEGF stimulus in HUVECs

| PRJNA436851 | ENA