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RNA sequencing of adult mouse ventricular cardiomyocytes incubated in normal growth medium or medium conditioned by CCR2+ cardiac macrophages

ABSTRACT: RNA-sequencing of primary cultured adult mouse cardiomyocytes under control conditions or cultured for 24h with medium conditioned by CCR2+ macrophages isolated from pressure overloaded mouse hearts

ORGANISM(S): Mus musculus

PROVIDER: GSE196798 | GEO | 2023/08/02

REPOSITORIES: GEO

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Project description:These are 3 TMT10 samples. The indicated "standard" sub-samples all refer to the same biological sample (obtained as a mixture of all other samples), split and tagged as indicated, to play as a reference for normalization. TMT10 sample1 contains the following sub-samples: -TAG 126: Standard. -TAG 127N: Day 3 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium. -TAG 127C: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium. -TAG 128N: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a VTN coating in RSeT medium. -TAG 128C: Not relevant. -TAG 129N: Not relevant. -TAG 129C: Not relevant. -TAG 130N: Not relevant. -TAG 130C: RSet medium. -TAG 131: Standard. TMT10 sample2 contains the following sub-samples: -TAG 126: Standard -TAG 127N: Day 3 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium. -TAG 127C: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium. -TAG 128N: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a VTN coating in RSeT medium. -TAG 128C: Not relevant. -TAG 129N: Day 2 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium. -TAG 129C: Day 2 conditioned medium of naive human pluripotent stem cells cultured on a VTN coating in RSeT medium. -TAG 130N: Not relevant. -TAG 130C: RSet medium. -TAG 131: Standard. TMT10 sample3 contains the following sub-samples: -TAG 126: Standard. -TAG 127N: Day 3 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium. -TAG 127C: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium. -TAG 128N: Day 2 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium. -TAG 128C: Day 2 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium. -TAG 129N: Day 2 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium. -TAG 129C: Day 2 conditioned medium of naive human pluripotent stem cells cultured on a VTN coating in RSeT medium. -TAG 130N: Not relevant. -TAG 130C: RSet medium. -TAG 131: Standard. Detailed culture and processing methods are described in Cesare et al, under submission.

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RNA sequencing of adult mouse ventricular cardiomyocytes incubated in normal growth medium or medium conditioned by CCR2+ cardiac macrophages

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