Transcriptomics

Dataset Information

0

Microglia coordinate cellular interactions during spinal cord repair


ABSTRACT: Traumatic spinal cord injury (SCI) triggers a neuro-inflammatory response dominated by tissue-resident microglia and monocyte derived macrophages (MDMs). Since activated microglia and MDMs are morphologically identical and express similar phenotypic markers in vivo, identifying injury responses specifically coordinated by microglia has historically been challenging. Here, we pharmacologically depleted microglia and use anatomical, histopathological, tract tracing, bulk and single cell RNA sequencing to reveal the cellular and molecular responses to SCI controlled by microglia. We show that microglia are vital for SCI recovery and coordinate injury responses in CNS-resident glia and infiltrating leukocytes. Depleting microglia exacerbates tissue damage and worsens functional recovery. Conversely, restoring select microglia-dependent signaling axes, identified through sequencing data, in microglia depleted mice prevents secondary damage and promotes recovery. Additional bioinformatics analyses reveal that optimal repair after SCI and likely other forms of neurological disease, might be achieved by co-opting key ligand-receptor interactions between microglia, astrocytes and MDMs.

ORGANISM(S): Mus musculus

PROVIDER: GSE196928 | GEO | 2022/06/01

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2018-08-01 | GSE102482 | GEO
2021-12-24 | GSE142849 | GEO
2020-04-07 | GSE129694 | GEO
2019-04-29 | GSE124819 | GEO
2024-04-29 | GSE242507 | GEO
2019-12-23 | GSE113566 | GEO
2007-09-04 | E-GEOD-464 | biostudies-arrayexpress
2012-01-01 | GSE29488 | GEO
2024-09-20 | GSE264511 | GEO
2018-09-25 | PXD010192 | Pride