Effect of nitrate on the transcriptome of the primary root tip in Medicago truncatula
Ontology highlight
ABSTRACT:
ORGANISM(S): Medicago truncatula
PROVIDER: GSE197021 | GEO | 2022/02/20
REPOSITORIES: GEO
Similar Datasets
Project description:The impact of nitrate on the proteome of the primary root tip of Medicago truncatula were compared in R108, a wild and sensitive genotype, and npf6.8, a mutant and insensitive line by shotgun analysis. Each genotype were cultivated in N-free condition or with 5mM of nitrate in three independent biological replicate. Sample collection was performed of 10 days old seedlings
2022-05-20 | PXD030547 | Pride
Project description:12plex_medicago_2013-08 - r108 permissive medium versus non permissive medium. - Two experiments to compare the transcriptomic response of medicago plants: Agar medium versus Phytagel medium (exp1) and rhizobium WT versus BacA (exp2). - Medicago truncatula R108 seedlings were inoculated with S. medicae WSM419 and were cultivated during three days on buffered nidulation medium solidified with Phytagel or Agar.
2018-11-01 | GSE121771 | GEO
Project description:12plex_medicago_2013-08 - r108 in symbiosis with rhizobia wt or rhizobia mutant for baca. - Two experiments to compare the transcriptomic response of medicago plants: Agar medium versus Phytagel medium (exp1) and rhizobium WT versus BacA (exp2). - Medicago truncatula ecotype R108 was inoculated with the symbiotic rhizobium Sinorhizobium meliloti strain Sm1021 and with its derivative mutant delta bacA. Nodules were collected 13 days after inoculation, and RNA were prepared for transcriptome analysis, there were three biological independant experiements.
2018-11-01 | GSE121775 | GEO
Project description:A transcriptome analysis of developing abi5 and wild type (R108) seeds from Medicago truncatula was performed to decipher the role of ABI54 in the regulation of late seed maturation and seed longevity.
2016-09-24 | GSE87313 | GEO
Project description:Desiccation tolerance (DT) is the capacity to withstand total loss of cellular water. This property is acquired during seed filling and lost just after germination. However, in many species, a germinated seed can regain DT under adverse conditions such as osmotic stress. We discovered that the germinated seeds of the abscisic acid insensitive 5 (Mtabi5) mutant of Medicago truncatula lost their ability to re-establish DT during osmotic stress. To characterize the molecular processes that are influenced by MtABI5 during the re-establishment of DT tolerance, a transcriptome analysis was performed on the protruded radicles of germinated Mtabi5 mutants and wilt type before and after an osmotic treatment. Six-condition experiment, R108_T versus R108_P, abi5-1_T versus abi5-1_P, abi5-2_T versus abi5-2_P. Biological replicates: 9 controls (R108-T, abi5-1_T, abi5-2_T), 9 treatments (R108-P, abi5-1_P, abi5-2_P), independently grown and harvested. One replicate per array.
2013-10-30 | E-GEOD-51830 | biostudies-arrayexpress
Project description:affy_root-dvt-nitrogen_medicago. The biological question is the study of root development adaptation to external nitrogen availability. One of the typical responses to low N provision consists of an increased root branching which promotes soil exploration. In order to differentiate between genes specifically involved in nitrate response and genes of root development, we had performed experiments on both a wild type and a mutant affected in root architecture, both supplied with two different levels of nitrate. Global gene expression profiling of root cells using microarray analysis will be conducted to identify genes expressed during root branching. First, analysing the differential gene expression between the wild type and the mutant both at low and high level of nitrate, we will identify genes of root development but also genes specifically involved in nitrate response. Then, additional comparisons will concern the modifications of wild type and mutant gene expression between the two levels of nitrate. These genes will be compared to those found to be expressed in the wild type roots under submitted to various nitrogen sources (Ruffel et al., 2008). All together these results will allow us to determine which genes, among the firstly underlined, are specifically involved in root development. The kinetics of expression in different tissues of the most relevant of these genes will be further analysed by qRT-PCR. Three successive experiments in growth chamber were performed on two different genotypes of Medicago truncatula. Each experiment constituted a biological replicate. For each of them, after 4 days of seed cold-treatment followed by 4 days of germination, individual plantlets were transferred onto hydroponic culture tanks containing vigorously aerated nutrient solution. Each tank contained 6 plantlets of J5 and 6 plantlets of the mutant. On one shelf of the growth chamber, nutrient solution in the tanks was supplemented by 1mM of KNO3; on the other shelf, the level of KNO3 in the solution was of 10mM. Nutrient solution was renewed every week. Roots of the two genotypes were harvested separately but at the same time, 10 days after the plantlets transfer onto hydroponic culture tanks. Keywords: dose response, wt vs mutant comparison
2010-01-29 | GSE18318 | GEO
Project description:Effect of nitrate on the transcriptome of the primary root tip in Medicago truncatula
| PRJNA808408 | ENA
Project description:affy_root-dvt-nitrogen_medicago. The biological question is the study of root development adaptation to external nitrogen availability. One of the typical responses to low N provision consists of an increased root branching which promotes soil exploration. In order to differentiate between genes specifically involved in nitrate response and genes of root development, we had performed experiments on both a wild type and a mutant affected in root architecture, both supplied with two different levels of nitrate. Global gene expression profiling of root cells using microarray analysis will be conducted to identify genes expressed during root branching. First, analysing the differential gene expression between the wild type and the mutant both at low and high level of nitrate, we will identify genes of root development but also genes specifically involved in nitrate response. Then, additional comparisons will concern the modifications of wild type and mutant gene expression between the two levels of nitrate. These genes will be compared to those found to be expressed in the wild type roots under submitted to various nitrogen sources (Ruffel et al., 2008). All together these results will allow us to determine which genes, among the firstly underlined, are specifically involved in root development. The kinetics of expression in different tissues of the most relevant of these genes will be further analysed by qRT-PCR. Three successive experiments in growth chamber were performed on two different genotypes of Medicago truncatula. Each experiment constituted a biological replicate. For each of them, after 4 days of seed cold-treatment followed by 4 days of germination, individual plantlets were transferred onto hydroponic culture tanks containing vigorously aerated nutrient solution. Each tank contained 6 plantlets of J5 and 6 plantlets of the mutant. On one shelf of the growth chamber, nutrient solution in the tanks was supplemented by 1mM of KNO3; on the other shelf, the level of KNO3 in the solution was of 10mM. Nutrient solution was renewed every week. Roots of the two genotypes were harvested separately but at the same time, 10 days after the plantlets transfer onto hydroponic culture tanks. Experiment Overall Design: 12 arrays - Medicago. Experiment Overall Design: WT and mutant (tr185) under 1mM or 10mM KNO3. Three biological replicates each genotype/condition.
2010-02-21 | E-GEOD-18318 | biostudies-arrayexpress
Project description:Medicago truncatula Tnt-1 insertional mutant for MtTTG1 gene (NF977) versus the wild-type R108. The seed samples are harvested at 16 days after pollination. Their transcript profiles were compared.
2009-08-14 | E-MEXP-1757 | biostudies-arrayexpress
Project description:To complement N2 fixation through symbiosis, legumes can efficiently acquire soil mineral N through adapted root architecture. However, root architecture adaptation to mineral N availability has been little studied in legumes. Therefore, this study investigated the effect of nitrate availability on root architecture in Medicago truncatula and assessed the N-uptake potential of a new highly branched root mutant, TR185. The effects of varying nitrate supply on both root architecture and N uptake were characterized in the mutant and in the wild type. Surprisingly, the root architecture of the mutant was not modified by variation in nitrate supply. Moreover, despite its highly branched root architecture, TR185 had a permanently N-starved phenotype. A transcriptome analysis was performed to identify genes differentially expressed between the two genotypes. This analysis revealed differential responses related to the nitrate acquisition pathway and confirmed that N starvation occurred in TR185. Changes in amino acid content and expression of genes involved in the phenylpropanoid pathway were associated with differences in root architecture between the mutant and the wild type.
| S-EPMC4036509 | biostudies-literature