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Ocular proteomic and transcriptomic changes with aging in response to lensectomy in a rabbit model of lensectomy with intraocular lens insertion

ABSTRACT: Previous work has established the use of a juvenile rabbit model and demonstrated an increased abundance of inflammatory and coagulation cascade proteins in the aqueous humor (AH) following lensectomy with IOL implantation at the time of surgery (Young et al., 2020).Expanding on this work, we seek to establish how the abundance of inflammatory and coagulation proteins in the anterior chamber changes with increasing age following lensectomy with IOL insertion in this rabbit animal model, seeking to explain the difference in post-operative scarring seen between children and adult humans.

ORGANISM(S): Oryctolagus cuniculus

PROVIDER: GSE197306 | GEO | 2022/02/26

REPOSITORIES: GEO

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Proteomic profiling of rabbit eyes

Project description:Rabbits have been widely used for studying ocular physiology and pathology due to their relatively large eye size and similar structures with human eyes. Various rabbit ocular disease models, such as dry eye, age-related macular degeneration, and glaucoma, have been established. Despite the growing application of proteomics in vision research using rabbit ocular models, there is no spectral assay library for rabbit eye proteome publicly available. Here, we generated spectral assay libraries for rabbit eye compartments, including conjunctiva, cornea, iris, retina, sclera, vitreous humor, and tears using fractionated samples and ion mobility separation enabling deep proteome coverage. The rabbit eye spectral assay library includes 9,153 protein groups, and 107,898 peptides. We present the data as a freely available community resource for proteomic studies in the vision field.

2021-11-24 | PXD029818 | Pride

Quantitative proteomic analysis of aqueous humor in a juvenile rabbit lensectomy model reveals surgery stage specific differences in coagulation, complement, and ocular immunosuppressive proteins

Project description:Purpose: To identify and quantify the proteins associated with the robust postoperative immune response in a juvenile rabbit model of lensectomy with intraocular lens (IOL) insertion. Methods: Twenty-six 6-7 week-old New Zealand White rabbits underwent unilateral portions of lensectomy with IOL insertion including: anterior chamber paracentesis, corneal incision with wound suture, lensectomy only, and lensectomy with IOL insertion. Aqueous humor (AH) was collected immediately prior and three days after each procedure. Semi-quantitative discovery of proteins was achieved using liquid chromatography tandem mass spectrometry (LC-MS/MS). Based on discovery results, targeted quantitation utilizing stable isotopically labeled (SIL) peptides for 3 proteins of interest, fibrinogen-beta chain, transforming growth factor beta-2, and retinol binding protein 3, were used to confirm the observed quantitative trends.

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Ocular proteomic and transcriptomic changes with aging in a rabbit model of lensectomy with intraocular lens insertion.

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Proteomic profiling of various rabbit eye tissues

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NMR-Based Metabolomics of Non-Human Primate Ocular Tissues: A Dataset

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Project description:Rabbit Lung_DH_TO_Control

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In vitro and in vivo models of Staphylococcus aureus endophthalmitis implicate specific nutrients in ocular infection

Project description:S. aureus SA564 and SA564-codY-mutant were grown in bovine aqueous humor, bovine vitreous humor and a chemically defined medium. Samples were extracted in midlog phase and affymetrix microarray processing was performed.

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