Endocrine, immune and renal toxicity in Largemouth bass after chronic exposure to glyphosate and Rodeo

ABSTRACT: Glyphosate is the herbicide most used worldwide, with no historic comparison. It is used for genetically modified crops, and particularly in Florida, it is used as a sugar cane ripener. It is also formulated to treat aquatic weeds in waterbodies as Rodeo®. Because of its extended use, it can run off or be sprayed directly into waterbodies and aquatic wildlife can be chronically exposed. Exposure in animal models has been associated with kidney and liver damage and it has been suggested as an endocrine disruptor. We exposed adult male largemouth bass for 21 days to two doses of glyphosate and Rodeo (chemically equivalent concentration of glyphosate) including 0.5 mg/L and 10 mg/L and to clean water as a control (n=4/tank in quadruplicate). Concentrations during the experiment were corroborated with LC-MS/MS. Total RNA was isolated from the trunk kidney and head kidney. RNA-seq was performed for the high doses compared to controls (n=4/treatment). Transcripts were analyzed with fish and mammalian pathway analyses. Transcripts mapped to Zebrafish metabolic pathways using Paintomics showed the most significantly enriched pathway was steroid hormone biosynthesis in the trunk kidney. Steroid hormones were measured in plasma by LC-MS/MS. Total androgens were significantly reduced at 0.5 mg/L of glyphosate and Rodeo compared to control. While 11-ketotestosterone and estrone concentration was significantly reduced in all doses. Our results show a possible down regulation of the conversion of testosterone to 11-ketotestosterone by glyphosate and Rodeo at concentrations that are allowed in U.S drinking water. Using the mammalian pathway analysis algorithm, cellular processes associated with T-cell activation/development and intracellular pH were significantly enriched in the trunk kidney after glyphosate and Rodeo exposure. Glyphosate and Rodeo produce endocrine disruption corroborated at the hormone and gene expression level. Rodeo and glyphosate share gene expression pathways, however, Rodeo had more pronounced effects in largemouth bass.

ORGANISM(S): Micropterus salmoides

PROVIDER: GSE197375 | GEO | 2022/04/20

REPOSITORIES: GEO